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验证数据展示

  • IF Staining of human placenta using 81066-1-RR

    IF Staining of human placenta using 81066-1-RR

    Immunofluorescent analysis of (4% PFA) fixed human placenta tissue using Band 3/ AE1 antibody (81066-1-RR, Clone: 3K21 ) at dilution of 1:1600 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IF Staining of human placenta using 81066-1-RR

    IF Staining of human placenta using 81066-1-RR

    Immunofluorescent analysis of (4% PFA) fixed human placenta tissue using Band 3/ AE1 antibody (81066-1-RR, Clone: 3K21 ) at dilution of 1:1600 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IHC staining of human placenta using 81066-1-RR

    IHC staining of human placenta using 81066-1-RR

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 81066-1-RR (SLC4A1 antibody) at dilution of 1:1700 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Band 3/AE1 antibody included
  • Affinity and Kinetic Characterization of 81066-1-RR

    Affinity and Kinetic Characterization of 81066-1-RR

    Biolayer interferometry (BLl) kinetic assays of 81066-1-RR against Human Band 3/AE 1 were performed. The affinity constant is 23.6 pM.

Band 3/AE 1 Recombinant monoclonal antibody

Band 3/AE 1 Uni-rAb® Recombinant Antibody for IHC, IF-P, ELISA
Cat No. 81066-1-RR

产品说明书

CloneNo. 3K21

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IHC, IF-P, ELISA

别名

Band 3 / AE1, band 3, band 3/ AE1, SLC4A1, Anion exchanger 1

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman placenta tissue
Planning an IHC experiment? We recommend our IHCeasy Band 3/AE1 Ready-To-Use IHC Kit. Band 3/AE1 primary antibody included.

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)-PIF-P : 1:800-1:3200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

81066-1-RR targets Band 3/AE 1 in IHC, IF-P, ELISA applications and shows reactivity with human samples.

经测试应用 IHC, IF-P, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag27878

Product name: Recombinant human SLC4A1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-200 aa of BC101570

Sequence: MEELQDDYEDMMEENLEQEEYEDPDIPESQMEEPAAHDTEATATDYHTTSHPGTHKVYVELQELVMDEKNQELRWMEAARWVQLEENLGENGAWGRPHLSHLTFWSLLELRRVFTKGTVLLDLQETSLAGVANQLLDRFIFEDQIRPQDREELLRALLLKHSHAGELEALGGVKPAVLTRSGDPSQPLLPQHSSLETQLF

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 solute carrier family 4, anion exchanger, member 1 (erythrocyte membrane protein band 3, Diego blood group)
别名 Band 3 / AE1, band 3, band 3/ AE1, SLC4A1, Anion exchanger 1
计算分子量 911 aa, 102 kDa
GenBank蛋白编号BC101570
基因名称 band 3
Gene ID (NCBI) 6521
RRIDAB_2935547
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP02730
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Anion exchanger 1 (AE1), also known as band 3, is a 95-100 kDa transmembrane glycoprotein which is abundantly expressed in erythrocyte plasma membrane and mediates the electroneutral exchange of Cl- and HCO3-. It contains a 52-55 kDa C-terminal membrane crossing domain and a 43 kDa N-terminal cytoplasmic domain. Degradation of band 3 protein occurred during erythrocyte aging. The oxidative stress can also activate the proteolysis of band 3 through caspases.

实验方案

Product Specific Protocols
IF protocol for Band 3/AE 1 antibody 81066-1-RRDownload protocol
IHC protocol for Band 3/AE 1 antibody 81066-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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