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验证数据展示

  • WB analysis using 20131-1-AP

    WB analysis using 20131-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 20131-1-AP (SLC43A3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary cancer using 20131-1-AP

    IHC staining of human ovary cancer using 20131-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 20131-1-AP (SLC43A3 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

SLC43A3 Polyclonal antibody

SLC43A3 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 20131-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

Protein FOAP 13, PRO1659, FOAP 13, Equilibrative nucleobase transporter 1, ENBT1

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHL-60 cells, MCF-7 cells
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20131-1-AP targets SLC43A3 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag13999

Product name: Recombinant human SLC43A3 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 211-278 aa of BC003163

Sequence: GHIPYPLPPNYSYGLCPGNGTTKEEKETAEHENRELQSKEFLSAKEETPGAGQKQELRSFWSYAFSRR

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 solute carrier family 43, member 3
别名 Protein FOAP 13, PRO1659, FOAP 13, Equilibrative nucleobase transporter 1, ENBT1
计算分子量 491 aa, 55 kDa
观测分子量55 kDa
GenBank蛋白编号BC003163
基因名称 SLC43A3
Gene ID (NCBI) 29015
RRIDAB_3085599
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8NBI5
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Sodium-independent purine-selective nucleobase transporter(SLC43A3), also called as equilibrative nucleobase transporter 1(ENBT1), is a nucleobase transporter involved in purine salvage pathways in mammals, which mediates the equilibrative transport of extracellular purine nucleobases such as adenine, guanine and hypoxanthine.(PubMed:26455426, PubMed:32339528) It has been found that it may regulate the transport of fatty acids (FA) in fat cells, as a positive regulator of FA efflux and as a negative regulator of FA uptake.(PMID: 32217606)

实验方案

Product Specific Protocols
IHC protocol for SLC43A3 antibody 20131-1-APDownload protocol
WB protocol for SLC43A3 antibody 20131-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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