验证数据展示
经过测试的应用
Positive WB detected in | HL-60 cells, MCF-7 cells |
Positive IHC detected in | human ovary cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:500-1:2000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
20131-1-AP targets SLC43A3 in WB, IHC, ELISA applications and shows reactivity with human samples.
经测试应用 | WB, IHC, ELISA Application Description |
经测试反应性 | human |
免疫原 | SLC43A3 fusion protein Ag13999 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | solute carrier family 43, member 3 |
别名 | Protein FOAP 13, PRO1659, FOAP 13, Equilibrative nucleobase transporter 1, ENBT1 |
计算分子量 | 491 aa, 55 kDa |
观测分子量 | 55 kDa |
GenBank蛋白编号 | BC003163 |
基因名称 | SLC43A3 |
Gene ID (NCBI) | 29015 |
RRID | AB_3085599 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | Q8NBI5 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Sodium-independent purine-selective nucleobase transporter(SLC43A3), also called as equilibrative nucleobase transporter 1(ENBT1), is a nucleobase transporter involved in purine salvage pathways in mammals, which mediates the equilibrative transport of extracellular purine nucleobases such as adenine, guanine and hypoxanthine.(PubMed:26455426, PubMed:32339528) It has been found that it may regulate the transport of fatty acids (FA) in fat cells, as a positive regulator of FA efflux and as a negative regulator of FA uptake.(PMID: 32217606)
实验方案
Product Specific Protocols | |
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WB protocol for SLC43A3 antibody 20131-1-AP | Download protocol |
IHC protocol for SLC43A3 antibody 20131-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |