验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, mouse liver tissue, mouse pancreas tissue |
| Positive IP detected in | mouse heart tissue |
| Positive IHC detected in | human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HEK-293 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
14315-1-AP targets SLC38A3 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, IP, CoIP |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag5553 Product name: Recombinant human SLC38A3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-191 aa of BC042875 Sequence: MEAPLQTEMVELVPNGKHSEGLLPVITPMAGNQRVEDPARSCMEGKSFLQKSPSKEPHFTDFEGKTSFGMSVFNLSNAIMGSGILGLAYAMANTGIILFLFLLTAVALLSSYSIHLLLKSSGVVGIRAYEQLGYRAFGTPGKLAAALAITLQNIGAMSSYLYIIKSELPLVIQTFLNLEEKTSDWYMNGNY 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | solute carrier family 38, member 3 |
| 别名 | G17, Na(+)-coupled neutral amino acid transporter 3, NAT1, N-system amino acid transporter 1, SN1 |
| 计算分子量 | 504 aa, 56 kDa |
| 观测分子量 | 65-70 kDa |
| GenBank蛋白编号 | BC042875 |
| 基因名称 | SLC38A3 |
| Gene ID (NCBI) | 10991 |
| RRID | AB_2254879 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q99624 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SLC38A3, also known as SNAT3 or SN1, belongs to the SLC38 family of sodium-coupled neutral amino acid transporters. SLC38A3 mediates glutamine transport with sodium and proton coupled manner, and it contributes to ammonia metabolism in the liver and brain. SLC38A3 is highly expressed in the liver and brain astrocytes, with modest expression in the kidney, heart, and muscle. SLC38A3 may migrate higher than the predicted 56 kDa due to the glycosylation.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for SLC38A3 antibody 14315-1-AP | Download protocol |
| IHC protocol for SLC38A3 antibody 14315-1-AP | Download protocol |
| IP protocol for SLC38A3 antibody 14315-1-AP | Download protocol |
| WB protocol for SLC38A3 antibody 14315-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Autophagy The HGF-MET axis coordinates liver cancer metabolism and autophagy for chemotherapeutic resistance. | ||
Cancer Lett Amino acid transporter SLC38A3 promotes metastasis of non-small cell lung cancer cells by activating PDK1. | ||
Cancer Lett RCCD1 depletion attenuates TGF-β-induced EMT and cell migration by stabilizing cytoskeletal microtubules in NSCLC cells. | ||
Cancer Lett Glutamine transporter SLC38A3 promotes breast cancer metastasis via Gsk3β/β-catenin/EMT pathway | ||
Int J Biol Sci Disrupted astrocyte-neuron glutamine-glutamate cycling in the medial prefrontal cortex contributes to depression-like behaviors.
| ||
Environ Pollut Long-term exposure changes the environmentally relevant bis(2-ethylhexyl) phthalate to be a neuro-hazardous substance disrupting neural homeostasis in emotional and cognitive functions |
