验证数据展示

WB analysis using 28632-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 28632-1-AP (SLC38A1 antibody) at dilution of 1:7000 incubated at room temperature for 1.5 hours.
FC experiment of A549 using 28632-1-AP
1x10^6 A549 cells were intracellularly stained with 0.25 ug SLC38A1 Polyclonal antibody (28632-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Intracellular Flow Cytometry Fixation & Permeabilization Buffer Kit (PF00019).
WB analysis of HeLa using 28632-1-AP
WB result of SLC38A1 antibody (28632-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SLC38A1 transfected Jurkat cells.
IHC staining of mouse brain using 28632-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 28632-1-AP (SLC38A1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with SLC38A1 antibody included
IHC staining of mouse brain using 28632-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 28632-1-AP (SLC38A1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with SLC38A1 antibody included
IHC staining of mouse cerebellum using 28632-1-AP
Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 28632-1-AP (SLC38A1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with SLC38A1 antibody included
IHC staining of human ovary cancer using 28632-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 28632-1-AP (SLC38A1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with SLC38A1 antibody included
IHC staining of human placenta using 28632-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 28632-1-AP (SLC38A1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with SLC38A1 antibody included
IHC staining of mouse lung using 28632-1-AP
Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 28632-1-AP (SLC38A1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with SLC38A1 antibody included

Featured Product
KD/KO Validated

SLC38A1 Polyclonal antibody

SLC38A1 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 28632-1-AP

产品说明书

引用文献(12)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse and More (1)

应用

WB, IHC, ELISA and More (1)

别名

Amino acid transporter A1, ATA1, NAT2, N-system amino acid transporter 2, SAT1

缓冲液配方: PBS, Azide, Glycerol

PBS, Azide, Glycerol

偶联物: Unconjugated

Unconjugated

规格	价格	库存

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概述

经过测试的应用
产品信息
发表文章 (12)
实验方案

产品说明书

MSDS

经过测试的应用

Positive WB detected in	A549 cells, mouse brain tissue, mouse skeletal muscle tissue
Positive IHC detected in	mouse brain tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive FC (Intra) detected in	A549 cells

Planning an IHC experiment? We recommend our IHCeasy SLC38A1 Ready-To-Use IHC Kit. SLC38A1 primary antibody included.

应用	推荐稀释比
Western Blot (WB)	WB : 1:2000-1:14000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

KD/KO	See 2 publications below
WB	See 12 publications below
IHC	See 4 publications below
IF	See 1 publications below

产品信息

28632-1-AP targets SLC38A1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

经测试应用	WB, IHC, ELISA Application Description
文献引用应用	WB, IHC, IF
经测试反应性	human, mouse
文献引用反应性	human, mouse, rat
免疫原	CatNo: Ag30088 Product name: Recombinant human SLC38A1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-74 aa of BC010620 Sequence: MMHFKSGLELTELQNMTVPEDDNISNDSNDFTEVENGQINSKFISDRESRRSLTNSHLEKKKCDEYIPGTTSLG 种属同源性预测
宿主/亚型	Rabbit / IgG
抗体类别	Polyclonal
产品类型	Antibody
全称	solute carrier family 38, member 1
别名	Amino acid transporter A1, ATA1, NAT2, N-system amino acid transporter 2, SAT1
计算分子量	487 aa, 54 kDa
观测分子量	54 kDa, 60-70 kDa
GenBank蛋白编号	BC010620
基因名称	SLC38A1
Gene ID (NCBI)	81539
RRID	AB_3086073
偶联类型	Unconjugated
形式	Liquid
纯化方式	Antigen affinity purification
UNIPROT ID	Q9H2H9
储存缓冲液	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

背景介绍

SLC38A1 (Sodium-coupled neutral amino acid transporter 1), is also named as SNAT1, which is a symporter that cotransports short-chain neutral amino acids and sodium ions from the extraccellular to the intracellular side of the cell membrane (PMID:20599747, PMID:10891391). Inhibition of SLC38A1 reduced tumor growth, cellular migration, invasion, and induced senescence in melanoma cells (PMID:35565278). SLC38A1 was identified as a positive regulator of mTORC1 in neurons, which promoted ischemic brain damage by mTOR-autophagy system (PMID:31552299). SLC38A1 is mainly expressed in brain and placenta.

实验方案

Product Specific Protocols
FC protocol for SLC38A1 antibody 28632-1-AP	Download protocol
IHC protocol for SLC38A1 antibody 28632-1-AP	Download protocol
WB protocol for SLC38A1 antibody 28632-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

发表文章

Species	Application	Title
mouse,human	WB	Metabolism ALDH2 deficiency aggravates vascular injury-induced restenosis by enhancing vascular smooth muscle cell proliferation through SLC38A2-mediated upregulation of glutamine uptake. Authors - Dehui Hou View Article
human, mouse	WB,IHC,IF	Cell Rep Med Nanoparticle-based serine replenishment rescues SP1-BNIP3 mitophagy and ameliorates preeclampsia in preclinical models. Authors - Yinan Wang View Article
mouse	WB	Sci Adv Liver regeneration-associated hepatocellular YAP1 activation prevents colorectal cancer liver metastasis through glutamine competition Authors - Qiang Yu View Article
human	WB,IHC	Cancer Lett Distinct Immunophenotypic Profiles and Neutrophil Heterogeneity in Colorectal Cancer Authors - Minghua Bai View Article
human	WB	Cancer Lett HHLA2 promotes immune evasion in EGFR-mutant lung cancer by inhibiting CD8(+) T cell glutamine metabolism via KIR3DL3 interaction. Authors - Fei Wu View Article
human	WB	Biomolecules Targeting Sodium Transport Reveals CHP1 Downregulation as a Novel Molecular Feature of Malignant Progression in Clear Cell Renal Cell Carcinoma: Insights from Integrated Multi-Omics Analyses Authors - Yun Wu View Article

验证数据展示

WB analysis using 28632-1-AP

FC experiment of A549 using 28632-1-AP

WB analysis of HeLa using 28632-1-AP

IHC staining of mouse brain using 28632-1-AP

IHC staining of mouse brain using 28632-1-AP

IHC staining of mouse cerebellum using 28632-1-AP

IHC staining of human ovary cancer using 28632-1-AP

IHC staining of human placenta using 28632-1-AP

IHC staining of mouse lung using 28632-1-AP

SLC38A1 Polyclonal antibody

SLC38A1 Polyclonal Antibody for WB, IHC, ELISA

经过测试的应用

推荐稀释比

发表文章中的应用

产品信息

背景介绍

实验方案

发表文章

ALDH2 deficiency aggravates vascular injury-induced restenosis by enhancing vascular smooth muscle cell proliferation through SLC38A2-mediated upregulation of glutamine uptake.

Nanoparticle-based serine replenishment rescues SP1-BNIP3 mitophagy and ameliorates preeclampsia in preclinical models.

Liver regeneration-associated hepatocellular YAP1 activation prevents colorectal cancer liver metastasis through glutamine competition

Distinct Immunophenotypic Profiles and Neutrophil Heterogeneity in Colorectal Cancer

HHLA2 promotes immune evasion in EGFR-mutant lung cancer by inhibiting CD8(+) T cell glutamine metabolism via KIR3DL3 interaction.

Targeting Sodium Transport Reveals CHP1 Downregulation as a Novel Molecular Feature of Malignant Progression in Clear Cell Renal Cell Carcinoma: Insights from Integrated Multi-Omics Analyses

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