验证数据展示
经过测试的应用
| Positive WB detected in | A549 cells, HEK-293 cells, PC-3 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
24066-1-AP targets SLC36A4 in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 | SLC36A4 fusion protein Ag21142 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | solute carrier family 36 (proton/amino acid symporter), member 4 |
| 别名 | PAT4, SLC36A4 |
| 计算分子量 | 504 aa, 56 kDa |
| 观测分子量 | 41-66 kDa |
| GenBank蛋白编号 | BC047374 |
| 基因名称 | SLC36A4 |
| Gene ID (NCBI) | 120103 |
| RRID | AB_2879424 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen Affinity purified |
| UNIPROT ID | Q6YBV0 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SLC36A4 (also known as proton-coupled amino-acid transporter 4, PAT4), is a member of the solute carrier (SLC) 36 family which functions as transporter for amino acids and derivatives. SLC36A4 is widely distributed at the mRNA level in normal tissues and many cancer cell lines. It modulates the mTORC1 signaling and is essential for the proliferation of cancer cells. In addition, small GTPase Rab12 controls the degradation of SLC36A4, which indirectly modulates mTORC1 activity and autophagy. This antibody can recognize the endogenous SLC36A4 protein in multiple cell lines.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for SLC36A4 antibody 24066-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
