验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells |
| Positive IHC detected in | human ovary cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 1 publications below |
产品信息
21295-1-AP targets SLC34A2 in WB, IHC, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human |
| 文献引用反应性 | human |
| 免疫原 | SLC34A2 fusion protein Ag15846 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | solute carrier family 34 (sodium phosphate), member 2 |
| 别名 | Na(+)/Pi cotransporter 2B, Na(+)-dependent phosphate cotransporter 2B, NaPi 2b, NAPI 3B, NAPI IIb |
| 计算分子量 | 690 aa, 76 kDa |
| 观测分子量 | 76 kDa |
| GenBank蛋白编号 | BC146666 |
| 基因名称 | SLC34A2 |
| Gene ID (NCBI) | 10568 |
| RRID | AB_2878835 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O95436 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SLC34A2, also known as NaPi-IIb, is a member of the solute carrier family 34 and encodes a type 2b sodium-dependent phosphate transporter. It is a multi-pass membrane protein composed of 690 amino acids. SLC34A2 is predominantly expressed in the lung, small intestine, and kidney. In the lung, it is specifically localized to the apical membrane of type II alveolar epithelial cells (ATII cells) and plays a crucial role during fetal lung development and embryonic development. It's reported that SLC34A2 is down-regulated in NSCLC tissues and cell lines. (PMID:26156586; 10329428)
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for SLC34A2 antibody 21295-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |