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验证数据展示

  • WB analysis of HeLa using 83844-5-RR

    WB analysis of HeLa using 83844-5-RR

    WB result of SLC31A1 antibody (83844-5-RR; 1:800; incubated at room temperature for 1.5 hours) with sh-Control and sh-SLC31A1 transfected HeLa cells.

  • WB analysis of SW480 using 83844-5-RR

    WB analysis of SW480 using 83844-5-RR

    WB result of SLC31A1 antibody (83844-5-RR; 1:800; room temperature for 1.5 hours) with wild-type and SLC31A1 knockout SW480 cells.

  • WB analysis using 83844-5-RR

    WB analysis using 83844-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83844-5-RR (SLC31A1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 83844-5-RR

    Affinity and Kinetic Characterization of 83844-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83844-5-RR against Human SLC31A1 were performed. The affinity constant is 3.33 nM.

  • Featured Product
  • KD/KO验证

SLC31A1 Recombinant antibody

SLC31A1 Uni-rAbTM Recombinant Antibody for WB, ELISA
Cat No. 83844-5-RR

产品说明书

CloneNo. 240868D10

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, ELISA

别名

COPT1, 240868D10, CTR1, Truncated CTR1 form

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, SW480 cells, HepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83844-5-RR targets SLC31A1 in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human
免疫原 SLC31A1 fusion protein Ag26601 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 solute carrier family 31 (copper transporters), member 1
别名 COPT1, 240868D10, CTR1, Truncated CTR1 form
计算分子量 21 kDa
观测分子量21-23 kDa
GenBank蛋白编号BC013611
基因名称 SLC31A1
Gene ID (NCBI) 1317
RRIDAB_3671429
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDO15431
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SLC31A1, also known as CTR1, COPT1 and solute carrier family 31 member 1, is a high-affinity copper transporter found in the cell membrane of human cells. It plays a crucial role in maintaining copper homeostasis within the body and functions as a homotrimer to influence dietary copper absorption in the cell membrane (PMID: 37853210; 28507097). SLC31A1 has three putative transmembrane domains, with an N-terminus located extracellularly and a C-terminus located intracellularly (PMID: 12827356). SLC31A1 can be detected a a 28 kDa band corresponding to glycosylated hCTR1 monomer, and a 24 kDa band that is unglycosylated hCTR1. In some membrane preparations a C-terminal degradation product of 17 kDa is also seen (PMID: 16135512). Additionally, SLC31A1 may also resolve as a 30 to 35 kDa and a 60 to 70 kDa protein on immunoblots, forming oligomers corresponding to the expected size of a homotrimeric complex (PMID: 12827356).

实验方案

Product Specific Protocols
WB protocol for SLC31A1 antibody 83844-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols