验证数据展示
经过测试的应用
| Positive WB detected in | HepG2 cells, HeLa cells |
| Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | human liver cancer tissue |
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:20000 |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
67221-1-Ig targets SLC31A1 in WB, IHC, IF-P, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, IF-P, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag28410 Product name: Recombinant human SLC31A1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-67 aa of BC013611 Sequence: MDHSHHMGMSYMDSNSTMQPSHHHPTTSASHSHGGGDSSMMMMPMTFYFGFKNVELLFSGLVINTAG 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG2b |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | solute carrier family 31 (copper transporters), member 1 |
| 别名 | COPT1, 1A4H5, CTR1, Truncated CTR1 form |
| 计算分子量 | 21 kDa |
| 观测分子量 | 32 kDa |
| GenBank蛋白编号 | BC013611 |
| 基因名称 | SLC31A1 |
| Gene ID (NCBI) | 1317 |
| RRID | AB_2882512 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | O15431 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for SLC31A1 antibody 67221-1-Ig | Download protocol |
| IF protocol for SLC31A1 antibody 67221-1-Ig | Download protocol |
| IHC protocol for SLC31A1 antibody 67221-1-Ig | Download protocol |
| WB protocol for SLC31A1 antibody 67221-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Front Mol Biosci Significance of cuproptosis- related genes in the diagnosis and classification of psoriasis | ||
J Virol Identification of Copper Transporter 1 as a Receptor for Feline Endogenous Retrovirus ERV-DC14.
| ||
Mol Biol Cell Adaptive protein synthesis in genetic models of copper deficiency and childhood neurodegeneration | ||
Front Immunol Plasma IgG and IgM autoantibodies to COPT1 as potential biomarkers for detection of non-small cell lung cancer | ||
BMC Cancer Copper's new role in cancer: how cuproptosis-related genes could revolutionize glioma treatment | ||
Front Immunol Identification of hub cuproptosis related genes and immune cell infiltration characteristics in periodontitis |
