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验证数据展示

  • WB analysis using 12623-1-AP

    WB analysis using 12623-1-AP

    LNCaP cells were subjected to SDS PAGE followed by western blot with 12623-1-AP (ZnT4 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 12623-1-AP

    IHC staining of mouse brain using 12623-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12623-1-AP (ZnT4 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 12623-1-AP

    IHC staining of mouse brain using 12623-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12623-1-AP (ZnT4 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

ZnT4 Polyclonal antibody

ZnT4 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 12623-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

SLC30A4, Probable proton-coupled zinc antiporter SLC30A4, ZnT 4, ZnT-4

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inLNCaP cells
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:16000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12623-1-AP targets ZnT4 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse
免疫原 ZnT4 fusion protein Ag3313 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 solute carrier family 30 (zinc transporter), member 4
别名 SLC30A4, Probable proton-coupled zinc antiporter SLC30A4, ZnT 4, ZnT-4
计算分子量 429 aa, 47 kDa
观测分子量47 kDa
GenBank蛋白编号BC026089
基因名称 ZNT4
Gene ID (NCBI) 7782
RRIDAB_3085398
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO14863
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ZnT4, also known as SLC30A4, belongs to the cation diffusion facilitator (CDF) transporter (TC 2.A.4) family and SLC30A subfamily. ZnT4 transports Zn into the trans-Golgi apparatus for lactose synthesis, and across the apical cell membrane for efflux from MECs into milk. ZnT4 is expressed in numerous tissues and is enriched in the prostate. ZnT4 encodes a protein with a molecular weight of 47 kDa (PMID: 26538236).

实验方案

Product Specific Protocols
WB protocol for ZnT4 antibody 12623-1-APDownload protocol
IHC protocol for ZnT4 antibody 12623-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
WB

Biochim Biophys Acta Gene Regul Mech

Transcriptional regulation of Znt family members znt4, znt5 and znt10 and their function in zinc transport in yellow catfish (Pelteobagrus fulvidraco)

Authors - Lu-Lu Liu
  • KD Validated
View Article