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ZNT4 Polyclonal antibody

ZNT4 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 12623-1-AP

Print datasheet

Synonyms

SLC30A4, Zinc transporter 4, ZnT 4, ZNT4, ZnT-4

验证数据展示

  • WB analysis using 12623-1-AP

    WB analysis using 12623-1-AP

    LNCaP cells were subjected to SDS PAGE followed by western blot with 12623-1-AP (ZNT4 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 12623-1-AP

    IHC staining of mouse brain using 12623-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12623-1-AP (ZNT4 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 12623-1-AP

    IHC staining of mouse brain using 12623-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12623-1-AP (ZNT4 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inLNCaP cells
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12623-1-AP targets ZNT4 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications IHC, WB, ELISA
Tested Reactivity human, mouse
Immunogen ZNT4 fusion protein Ag3313 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name solute carrier family 30 (zinc transporter), member 4
Synonyms SLC30A4, Zinc transporter 4, ZnT 4, ZNT4, ZnT-4
Calculated Molecular Weight 429 aa, 47 kDa
Observed Molecular Weight47 kDa
GenBank Accession NumberBC026089
Gene Symbol SLC30A4
Gene ID (NCBI) 7782
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO14863
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ZNT4, also known as SLC30A4, belongs to the cation diffusion facilitator (CDF) transporter (TC 2.A.4) family and SLC30A subfamily. ZNT4 transports Zn into the trans-Golgi apparatus for lactose synthesis, and across the apical cell membrane for efflux from MECs into milk. ZNT4 is expressed in numerous tissues and is enriched in the prostate. ZNT4 encodes a protein with a molecular weight of 47 kDa (PMID: 26538236).

实验方案

Product Specific Protocols
WB protocol for ZNT4 antibody 12623-1-APDownload protocol
IHC protocol for ZNT4 antibody 12623-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols