验证数据展示
经过测试的应用
| Positive WB detected in | unboiled HEK-293 cells, 37°C incubated HeLa cells |
| Positive FC (Intra) detected in | HeLa cells |
For optimal WB detection with 81463-1-RR, we recommend to avoid boiling the sample after lysis.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 8 publications below |
| IHC | See 2 publications below |
产品信息
81463-1-RR targets GLUT1 in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, FC (Intra), ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse, chicken |
| 免疫原 |
CatNo: Ag16282 Product name: Recombinant human SLC2A1,GLUT1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 216-280 aa of BC121804 Sequence: INRNEENRAKSVLKKLRGTADVTHDLQEMKEESRQMMREKKVTILELFRSPAYRQPILIAVVLQL 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | solute carrier family 2 (facilitated glucose transporter), member 1 |
| 别名 | SLC2A1, GLUT-1, SLC2A1,GLUT1 |
| 计算分子量 | 492 aa, 54 kDa |
| 观测分子量 | 45-55 kDa |
| GenBank蛋白编号 | BC121804 |
| 基因名称 | GLUT1 |
| Gene ID (NCBI) | 6513 |
| RRID | AB_2923718 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P11166 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
GLUT1, also known as SLC2A1, is a ubiquitously expressed glucose transporter and is responsible for the basal level of glucose uptake in most cell types. Human erythrocytes express the highest level of GLUT1. Defects in SLC2A1 are the cause of GLUT1 deficiency syndrome type 1 and type 2. High expression of GLUT1 has been reported to be a reliable immunohistochemical marker for juvenile hemangiomas. GLUT1 protein may appear as two or more distinct forms among 43 kDa to 55 kDa due to the different glycosylation states. And the conversion of the highly glycosylated form of GLUT1 to a less glycosylated form has been reported to correlate with differentiation (PMID: 8263524, 23302780).
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for GLUT1 antibody 81463-1-RR | Download protocol |
| WB protocol for GLUT1 antibody 81463-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Int J Biol Macromol Glyphosate targets FYN to regulate glycolysis and promote glioblastoma proliferation: A network toxicology study | ||
Cytojournal Ubiquitin C-terminal hydrolase L5 promotes the development of renal cell carcinoma through the glycolysis mediated by the phosphatidylinositol-3-kinase/protein kinase B/mammalian target of rapamycin pathway.
| ||
Am J Pathol Cullin 2 Elevates the Warburg Effect to Accelerate the Development of Hypoxic Pulmonary Hypertension. | ||
Hum Cell Leucyl-tRNA synthetase promotes malignant progression in diffuse large B-cell lymphoma by regulating glycolysis via the LRPPRC/HIF-1α/HK2 axis. |
