验证数据展示
经过测试的应用
| Positive WB detected in | HepG2 cells, mouse liver tissue, SMMC-7721 cells, mouse colon tissue, rat colon tissue, rat liver tissue |
| Positive IP detected in | HepG2 cells |
| Positive IHC detected in | human ovary tumor tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:300-1:1200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
11013-1-AP targets SLC27A4/FATP4 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat, pig |
| 免疫原 |
CatNo: Ag1490 Product name: Recombinant human SLC27A4 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-237 aa of BC009959 Sequence: MPLTLSTLLQPGRIWTGRRAAEPTPGHNAAWSLSGGGAAVLQAGAETALDPGGILPVVPLLGIWRLALHPGLHQDHQAYLTGDVLVMDELGYLYFRDRTGDTFRWKGENVSTTEVEGTLSRLLDMADVAVYGVEVPGTEGRAGMAAVASPTGNCDLERFAQVLEKELPLYARPIFLRLLPELHKTGTYKFQKTELRKEGFDPAIVKDPLFYLDAQKGRYVPLDQEAYSRIQAGEEKL 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | solute carrier family 27 (fatty acid transporter), member 4 |
| 别名 | FATP 4, SLC27A4, ACSVL4, Arachidonate--CoA ligase, EC:6.2.1.- |
| 计算分子量 | 72 kDa |
| 观测分子量 | 70 kDa |
| GenBank蛋白编号 | BC009959 |
| 基因名称 | FATP4/SLC27A4 |
| Gene ID (NCBI) | 10999 |
| RRID | AB_2190637 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q6P1M0 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferPHVal}} |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for SLC27A4/FATP4 antibody 11013-1-AP | Download protocol |
| IP protocol for SLC27A4/FATP4 antibody 11013-1-AP | Download protocol |
| WB protocol for SLC27A4/FATP4 antibody 11013-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Metab Liver-breast communication of adipocyte-oriented exosomes drives primary mammary cancer progression | ||
Immunity Gasdermin-D-mediated epithelial-immune circuit synchronizes nutrient absorption and host defense in the small intestine | ||
Nat Commun Galactooligosaccharides and Limosilactobacillus reuteri synergistically alleviate gut inflammation and barrier dysfunction by enriching Bacteroides acidifaciens for pentadecanoic acid biosynthesis | ||
Exp Hematol Oncol NAT10-mediated lipid metabolic reprogramming drives EGFR-TKI resistance in non-small cell lung cancer via ac4C-dependent mRNA stabilization.
| ||
Cell Death Dis B-cell lymphoma 6 alleviates nonalcoholic fatty liver disease in mice through suppression of fatty acid transporter CD36. | ||
Phytomedicine Atractylodin ameliorates obesity-associated hepatic steatosis by regulating the PLIN2-ATGL/CPT1A axis-mediated lipid droplet-mitochondria interactions. |
