验证数据展示
经过测试的应用
| Positive WB detected in | human testis tissue, mouse testis tissue, MCF-7 cells |
| Positive IP detected in | mouse testis tissue |
| Positive IHC detected in | human skin cancer tissue, human breast cancer tissue, mouse skin tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 6 publications below |
| IHC | See 1 publications below |
产品信息
12943-1-AP targets SLC27A3 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IP, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse, pig |
| 免疫原 |
CatNo: Ag4015 Product name: Recombinant human SLC27A3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 500-700 aa of BC029792 Sequence: GEPGLLVAPVSQQSPFLGYAGGPELAQGKLLKDVFRPGDVFFNTGDLLVCDDQGFLRFHDRTGDTFRWKGENVATTEVAEVFEALDFLQEVNVYGVTVPGHEGRAGMAALVLRPPHALDLMQLYTHVSENLPPYARPRFLRLQESLATTETFKQQKVRMANEGFDPSTLSDPLYVLDQAVGAYLPLTTARYSALLAGNLRI 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | solute carrier family 27 (fatty acid transporter), member 3 |
| 别名 | ACSVL3, FATP 3, FATP3, Fatty acid transport protein 3, SLC27A3, UNQ367/PRO703, VLCS 3 |
| 计算分子量 | 75 kDa |
| 观测分子量 | 70-79 kDa |
| GenBank蛋白编号 | BC029792 |
| 基因名称 | SLC27A3 |
| Gene ID (NCBI) | 11000 |
| RRID | AB_2286200 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q5K4L6 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SLC27A3, also named as FATP3, is a member of the solute carrier 27A (SLC27A) gene family which encodes fatty acid transport proteins. SLC27A3 is expressed in human neural stem cells and plays a key role in the developmental stage of the central nervous system. It is associated with autism spectrum disorders (ASD) that caused by impaired early brain development.(PMID: 26548558).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for SLC27A3 antibody 12943-1-AP | Download protocol |
| IHC protocol for SLC27A3 antibody 12943-1-AP | Download protocol |
| IP protocol for SLC27A3 antibody 12943-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cancers (Basel) In Vivo Optical Metabolic Imaging of Long-Chain Fatty Acid Uptake in Orthotopic Models of Triple-Negative Breast Cancer. | ||
Eur J Pharmacol A novel therapy for hepatic cholestasis treatment-the combination of rosiglitazone and fenofibrate | ||
Front Immunol Machine-Learning Algorithm-Based Prediction of Diagnostic Gene Biomarkers Related to Immune Infiltration in Patients With Chronic Obstructive Pulmonary Disease. | ||
Mol Reprod Dev Cloned pig fetuses exhibit fatty acid deficiency from impaired placental transport. | ||
Int J Mol Sci Knockdown of VEGFB/VEGFR1 Signaling Promotes White Adipose Tissue Browning and Skeletal Muscle Development |
