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FATP2 Polyclonal antibody

FATP2 Polyclonal Antibody for IF/ICC, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, IHC, IP, WB, ELISA and More (1)

Conjugate

Unconjugated

Publications

Cat No : 14048-1-AP

Print datasheet

Synonyms

ACSVL1, FACVL1, FATP 2, FATP2, Fatty acid transport protein 2, hFACVL1, SLC27A2, THCA CoA ligase, VLACS, VLCS

验证数据展示

WB analysis using 14048-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 14048-1-AP (FATP2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis using 14048-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 14048-1-AP (FATP2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
IP experiment of HepG2 using 14048-1-AP
IP result of anti-FATP2 (IP:14048-1-AP, 4ug; Detection:14048-1-AP 1:500) with HepG2 cells lysate 3200ug.
IF Staining of HepG2 using 14048-1-AP
Immunofluorescent analysis of HepG2 cells using 14048-1-AP (SLC27A2 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IHC staining of human kidney using 14048-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14048-1-AP (FATP2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human kidney using 14048-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14048-1-AP (FATP2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 14048-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14048-1-AP (FATP2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 14048-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14048-1-AP (FATP2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Cat No. 14048-1-AP

Size

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经过测试的应用

Positive WB detected in	mouse kidney tissue, mouse liver, rat kidney, rat liver, mouse liver tissue, rat liver tissue
Positive IP detected in	HepG2 cells
Positive IHC detected in	human liver cancer tissue, human kidney tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF detected in	HepG2 cells

Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:16000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:250-1:1000
Immunofluorescence (IF)	IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

KD/KO	See 3 publications below
WB	See 30 publications below
IHC	See 2 publications below
IF	See 4 publications below
CoIP	See 1 publications below

产品信息

14048-1-AP targets FATP2 in WB, IP, IF, IHC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications	IF/ICC, IHC, IP, WB, ELISA
Cited Applications	WB, IF, IHC, CoIP
Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Immunogen	FATP2 fusion protein Ag5217 种属同源性预测
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Full Name	solute carrier family 27 (fatty acid transporter), member 2
Synonyms	ACSVL1, FACVL1, FATP 2, FATP2, Fatty acid transport protein 2, hFACVL1, SLC27A2, THCA CoA ligase, VLACS, VLCS
Calculated Molecular Weight	567 aa, 65 kDa
Observed Molecular Weight	70 kDa
GenBank Accession Number	BC057770
Gene Symbol	SLC27A2
Gene ID (NCBI)	11001
RRID	AB_2239416
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	O14975
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

背景介绍

FATP2 is a member of the FATP family which functions in lipid and bile metabolism. It is a 70-kDa protein predominantly expressed in liver and kidney. Kidney FATP2 is localized exclusively to proximal tubule epithelial cells along the apical but not the basolateral membrane, and regulates lipoapoptosis. FATP2 is involved in metabolism-related diseases including nonalcoholic fatty liver disease (NAFLD) and type 2 diabetes mellitus (T2DM), and is a potential clinical biomarker and therapeutic target.

实验方案

Product Specific Protocols
WB protocol for FATP2 antibody 14048-1-AP	Download protocol
IHC protocol for FATP2 antibody 14048-1-AP	Download protocol
IF protocol for FATP2 antibody 14048-1-AP	Download protocol
IP protocol for FATP2 antibody 14048-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

发表文章

Species	Application	Title
mouse	WB	Hepatology Letter to the editor: Adipose lipolysis is important for ethanol to induce fatty liver in the NIAAA murine model of chronic and binge ethanol feeding Authors - Wenhao Luo View Article
mouse	WB	Hepatology Adipose lipolysis is important for ethanol to induce fatty liver in the National Institute on Alcohol Abuse and Alcoholism murine model of chronic and binge ethanol feeding. Authors - Mallika Mathur View Article
rat	WB	Environ Pollut Hepatotoxicity induced in rats by chronic exposure to F-53B, an emerging replacement of perfluorooctane sulfonate (PFOS) Authors - Xiaohan Li View Article
human	WB	EMBO Rep Lin28 enhances de novo fatty acid synthesis to promote cancer progression via SREBP-1. Authors - Yang Zhang View Article
human	WB	Anal Chem Ionic Liquid-Based Extraction System for In-Depth Analysis of Membrane Protein Complexes. Authors - Hongwei Chu View Article
mouse	WB	Nephrol Dial Transplant Key enzyme in charge of ketone reabsorption of renal tubular SMCT1 may be a new target in diabetic kidney disease Authors - Zhenhong Guo View Article

FATP2 Polyclonal antibody

FATP2 Polyclonal Antibody for IF/ICC, IHC, IP, WB, ELISA

验证数据展示

WB analysis using 14048-1-AP

WB analysis using 14048-1-AP

IP experiment of HepG2 using 14048-1-AP

IF Staining of HepG2 using 14048-1-AP

IHC staining of human kidney using 14048-1-AP

IHC staining of human kidney using 14048-1-AP

IHC staining of human liver cancer using 14048-1-AP

IHC staining of human liver cancer using 14048-1-AP

经过测试的应用

推荐稀释比

发表文章中的应用

产品信息

背景介绍

实验方案

发表文章

Letter to the editor: Adipose lipolysis is important for ethanol to induce fatty liver in the NIAAA murine model of chronic and binge ethanol feeding

Adipose lipolysis is important for ethanol to induce fatty liver in the National Institute on Alcohol Abuse and Alcoholism murine model of chronic and binge ethanol feeding.

Hepatotoxicity induced in rats by chronic exposure to F-53B, an emerging replacement of perfluorooctane sulfonate (PFOS)

Lin28 enhances de novo fatty acid synthesis to promote cancer progression via SREBP-1.

Ionic Liquid-Based Extraction System for In-Depth Analysis of Membrane Protein Complexes.

Key enzyme in charge of ketone reabsorption of renal tubular SMCT1 may be a new target in diabetic kidney disease