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SLC26A2 Polyclonal antibody

SLC26A2 Polyclonal Antibody for FC, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat and More (2)

Applications

FC, IHC, WB, ELISA and More (1)

Conjugate

Unconjugated

Cat No : 27759-1-AP

Print datasheet

Synonyms

D5S1708, Diastrophic dysplasia protein, DTD, DTDST, EDM4, MST153, MSTP157, SLC26A2, Sulfate transporter

验证数据展示

  • WB analysis of mouse colon using 27759-1-AP

    WB analysis of mouse colon using 27759-1-AP

    mouse colon tissue were subjected to SDS PAGE followed by western blot with 27759-1-AP (SLC26A2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon using 27759-1-AP

    IHC staining of human colon using 27759-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 27759-1-AP (SLC26A2 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • FC experiment of U2OS using 27759-1-AP

    FC experiment of U2OS using 27759-1-AP

    1X10^6 U2OS cells were intracellularly stained with 0.4 ug Anti-Human SLC26A2 (27759-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IHC staining of human colon using 27759-1-AP

    IHC staining of human colon using 27759-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 27759-1-AP (SLC26A2 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inmouse colon tissue, rat colon tissue
Positive IHC detected inhuman colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inU2OS cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:2000-1:8000
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27759-1-AP targets SLC26A2 in WB, IF, FC, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications FC, IHC, WB, ELISA
Cited ApplicationsWB, IF
Tested Reactivity Human, mouse, rat
Cited Reactivityhuman, mouse, canine
Immunogen SLC26A2 fusion protein Ag26521 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name solute carrier family 26 (sulfate transporter), member 2
Synonyms D5S1708, Diastrophic dysplasia protein, DTD, DTDST, EDM4, MST153, MSTP157, SLC26A2, Sulfate transporter
Calculated Molecular Weight 82 kDa
Observed Molecular Weight 68 kDa
GenBank Accession NumberBC059390
Gene Symbol SLC26A2
Gene ID (NCBI) 1836
RRIDAB_2880963
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP50443
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for SLC26A2 antibody 27759-1-APDownload protocol
IHC protocol for SLC26A2 antibody 27759-1-APDownload protocol
FC protocol for SLC26A2 antibody 27759-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

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human,mouse,canineIF

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humanWB

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