验证数据展示
经过测试的应用
| Positive WB detected in | Jurkat cells, mouse brain tissue, rat brain tissue, MCF-7 cells |
| Positive IHC detected in | human stomach tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy SLC25A46 Ready-To-Use IHC Kit. SLC25A46 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
12277-1-AP targets SLC25A46 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag2923 Product name: Recombinant human SLC25A46 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-264 aa of BC017169 Sequence: MHPRRPDGFDGLGYRGGARDEQGFGGAFPARSFSTGSDLGHWVTTPPDIPGSRNLHWGEKSPPYGVPTTSTPYEGPTEEPFSSGGGGSVQGQSSEQLNRFAGFGIGLASLFTENVLAHPCIVLRRQCQVNYHAQHYHLTPFTVINIMYSFNKTQGPRALWKGMGSTFIVQGVTLGAEGIISEFTPLPREVLHKWSPKQIGEHLLLKSLTYVVAMPFYSASLIETVQSEIIRDNTGILECVKEGIGRVIGMGVPHSKRLLPLLSL 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | solute carrier family 25, member 46 |
| 别名 | Mitochondrial outer membrane protein SLC25A46, TB1 |
| 计算分子量 | 418 aa, 46 kDa |
| 观测分子量 | 46 kDa |
| GenBank蛋白编号 | BC017169 |
| 基因名称 | SLC25A46 |
| Gene ID (NCBI) | 91137 |
| RRID | AB_2239384 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q96AG3 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SLC25A46 belongs to the SLC25 family of mitochondrial carrier proteins.
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for SLC25A46 antibody 12277-1-AP | Download protocol |
| WB protocol for SLC25A46 antibody 12277-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Sci Adv Global ubiquitylation analysis of mitochondria in primary neurons identifies endogenous Parkin targets following activation of PINK1. | ||
EMBO Mol Med SLC25A46 is required for mitochondrial lipid homeostasis and cristae maintenance and is responsible for Leigh syndrome.
| ||
J Pers Med Reanalysis of Exome Data Identifies Novel SLC25A46 Variants Associated with Leigh Syndrome. | ||
Mol Biol Cell Rapid degradation of mutant SLC25A46 by the ubiquitin-proteasome system results in MFN1/2 mediated hyperfusion of mitochondria.
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