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验证数据展示

  • WB analysis using 20615-1-AP

    WB analysis using 20615-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 20615-1-AP (SLC25A40 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • IHC staining of human placenta using 20615-1-AP

    IHC staining of human placenta using 20615-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 20615-1-AP (SLC25A40 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human placenta using 20615-1-AP

    IHC staining of human placenta using 20615-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 20615-1-AP (SLC25A40 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse kidney using 20615-1-AP

    IHC staining of mouse kidney using 20615-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 20615-1-AP (SLC25A40 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

SLC25A40 Polyclonal antibody

SLC25A40 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 20615-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

MCFP

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inC2C12 cells, mouse kidney tissue, mouse liver tissue
Positive IHC detected inhuman placenta tissue, mouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:12000
Immunohistochemistry (IHC)IHC : 1:100-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20615-1-AP targets SLC25A40 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse
免疫原 SLC25A40 fusion protein Ag14669 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 solute carrier family 25, member 40
别名 MCFP
计算分子量 338 aa, 38 kDa
观测分子量30~38 kDa
GenBank蛋白编号BC027322
基因名称 SLC25A40
Gene ID (NCBI) 55972
RRIDAB_3669338
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8TBP6
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SLC25A40 (solute carrier family 25, member 40) is one of the solute carrier (SLC) transporters that comprise a family of MCS transporters and uptakes GSH into mitochondria (PMID: 37407483). The SLC25A40 mRNA levels were significantly increased in both the kidneys of LPS mice and KMRC-1 cells after LPS treatment, when compared with those in the controls (PMID: 35955707).

实验方案

Product Specific Protocols
WB protocol for SLC25A40 antibody 20615-1-APDownload protocol
IHC protocol for SLC25A40 antibody 20615-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols