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验证数据展示

  • WB analysis using 20615-1-AP

    WB analysis using 20615-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 20615-1-AP (SLC25A40 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • IHC staining of human placenta using 20615-1-AP

    IHC staining of human placenta using 20615-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 20615-1-AP (SLC25A40 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human placenta using 20615-1-AP

    IHC staining of human placenta using 20615-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 20615-1-AP (SLC25A40 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse kidney using 20615-1-AP

    IHC staining of mouse kidney using 20615-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 20615-1-AP (SLC25A40 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

SLC25A40 Polyclonal antibody

SLC25A40 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 20615-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA and More (1)

别名

MCFP

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inC2C12 cells, mouse kidney tissue, mouse liver tissue
Positive IHC detected inhuman placenta tissue, mouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:12000
Immunohistochemistry (IHC)IHC : 1:100-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20615-1-AP targets SLC25A40 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB, IHC, IF
经测试反应性 human, mouse
文献引用反应性human, mouse
免疫原

CatNo: Ag14669

Product name: Recombinant human SLC25A40 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-110 aa of BC027322

Sequence: MDPETRGQEIIKVTPLQQMLASCTGAILTSVIVTPLDVVKIRLQAQNNPLPKGKCFVYSNGLMDHLCVCEEGGNKLWYKKPGNFQGTLDAFFKIIRNEGIKSLWSGLPPT

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 solute carrier family 25, member 40
别名 MCFP
计算分子量 338 aa, 38 kDa
观测分子量30~38 kDa
GenBank蛋白编号BC027322
基因名称 SLC25A40
Gene ID (NCBI) 55972
RRIDAB_3669338
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8TBP6
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SLC25A40 (solute carrier family 25, member 40) is one of the solute carrier (SLC) transporters that comprise a family of MCS transporters and uptakes GSH into mitochondria (PMID: 37407483). The SLC25A40 mRNA levels were significantly increased in both the kidneys of LPS mice and KMRC-1 cells after LPS treatment, when compared with those in the controls (PMID: 35955707).

实验方案

Product Specific Protocols
IHC protocol for SLC25A40 antibody 20615-1-APDownload protocol
WB protocol for SLC25A40 antibody 20615-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
human, mouseWB,IHC,IF

Exp Cell Res

SLC25A40 promotes NSCLC growth by enhancing NADPH-mediated lipid synthesis and suppressing ROS accumulation-induced ferroptosis.

Authors - Peini Xue
  • KD Validated
View Article
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