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SLC25A4 Polyclonal antibody

SLC25A4 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 30631-1-AP

Print datasheet

Synonyms

AAC1, ADP,ATP carrier protein 1, ADP/ATP translocase 1, ANT, ANT 1, ANT1, PEO2, PEO3, SLC25A4, T1

验证数据展示

  • WB analysis using 30631-1-AP

    WB analysis using 30631-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30631-1-AP (SLC25A4 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse stomach using 30631-1-AP

    IHC staining of mouse stomach using 30631-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 30631-1-AP (SLC25A4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inC2C12 cells, mouse brain tissue, mouse heart tissue, mouse skeletal muscle tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30631-1-AP targets SLC25A4 in WB, ELISA applications and shows reactivity with Human, mouse samples.

Tested Applications IHC, WB, ELISA
Tested Reactivity Human, mouse
Immunogen SLC25A4 fusion protein Ag33385 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name solute carrier family 25 (mitochondrial carrier; adenine nucleotide translocator), member 4
Synonyms AAC1, ADP,ATP carrier protein 1, ADP/ATP translocase 1, ANT, ANT 1, ANT1, PEO2, PEO3, SLC25A4, T1
Calculated Molecular Weight 33 kDa
Observed Molecular Weight33 kDa
GenBank Accession NumberBC008664
Gene Symbol SLC25A4
Gene ID (NCBI) 291
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP12235
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Adenine nucleotide translocators (ANTs) are mitochondrial carrier proteins that exchange ATP and ADP between the cytoplasm and the mitochondrial matrix. Four isoforms of ANT were identified: ANT1 (encoded by SLC25A4) is highly expressed in different tissues; ANT2 (encoded by SLC25A5) is predominantly expressed in proliferating, regenerating, or undifferentiated cells; ANT3 is ubiquitously expressed; ANT4 is mainly expressed in testis and germ cells. This antibody recognizes both of ANT1 and ANT2.

实验方案

Product Specific Protocols
WB protocol for SLC25A4 antibody 30631-1-APDownload protocol
IHC protocol for SLC25A4 antibody 30631-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols