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验证数据展示

  • WB analysis using 33263-1-AP

    WB analysis using 33263-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 33263-1-AP (ANT4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. Negative control: mouse brain tissue, mouse heart tissue.

  • IHC staining of mouse testis using 33263-1-AP

    IHC staining of mouse testis using 33263-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 33263-1-AP (SLC25A31/ANT4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse testis using 33263-1-AP

    IHC staining of mouse testis using 33263-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 33263-1-AP (SLC25A31/ANT4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse kidney using 33263-1-AP

    IHC staining of mouse kidney using 33263-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 33263-1-AP (SLC25A31/ANT4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse kidney using 33263-1-AP

    IHC staining of mouse kidney using 33263-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 33263-1-AP (SLC25A31/ANT4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of U-251 using 33263-1-AP

    IF Staining of U-251 using 33263-1-AP

    Immunofluorescent analysis of (4% PFA) fixed U-251 cells using SLC25A31/ANT4 antibody (33263-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

SLC25A31/ANT4 Polyclonal antibody

SLC25A31/ANT4 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 33263-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

ANT4, SLC25A31, AAC4, Adenine nucleotide translocator 4, ADP,ATP carrier protein 4

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inhuman testis tissue, mouse testis tissue, rat testis tissue
Positive IHC detected inmouse kidney tissue, mouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU-251 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

33263-1-AP targets SLC25A31/ANT4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag11008

Product name: Recombinant human SLC25A31 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-315 aa of BC022032

Sequence: MHREPAKKKAEKRLFDASSFGKDLLAGGVAAAVSKTAVAPIERVKLLLQVQASSKQISPEARYKGMVDCLVRIPREQGFFSFWRGNLANVIRYFPTQALNFAFKDKYKQLFMSGVNKEKQFWRWFLANLASGGAAGATSLCVVYPLDFARTRLGVDIGKGPEERQFKGLGDCIMKIAKSDGIAGLYQGFGVSVQGIIVYRASYFGAYDTVKGLLPKPKKTPFLVSFFIAQVVTTCSGILSYPFDTVRRRMMMQSGEAKRQYKGTLDCFVKIYQHEGISSFFRGAFSNVLRGTGGALVLVLYDKIKEFFHIDIGGR

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 solute carrier family 25 (mitochondrial carrier; adenine nucleotide translocator), member 31
别名 ANT4, SLC25A31, AAC4, Adenine nucleotide translocator 4, ADP,ATP carrier protein 4
计算分子量 315 aa, 35 kDa
观测分子量 35 kDa
GenBank蛋白编号BC022032
基因名称 SLC25A31
Gene ID (NCBI) 83447
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ9H0C2
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SLC25A31, also known as ANT4, is a member of the mitochondrial carrier family. The proteins of this family are mainly involved in the transport of substances inside and outside the mitochondria. SLC25A31 is an ADP/ATP antiporter protein, which catalyzes the exchange of ATP generated in mitochondria by ATP synthase against ADP produced in cytosol by most energy-consuming reactions(PMID: 15670820). SLC25A31 is mainly expressed in the testis and male germ cells (PMID: 25031318, 23506884).

实验方案

Product Specific Protocols
IF protocol for SLC25A31/ANT4 antibody 33263-1-APDownload protocol
IHC protocol for SLC25A31/ANT4 antibody 33263-1-APDownload protocol
WB protocol for SLC25A31/ANT4 antibody 33263-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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