验证数据展示
经过测试的应用
| Positive WB detected in | mouse brain tissue, rat brain tissue |
| Positive IHC detected in | human pancreas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
26663-1-AP targets SLC25A29 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human |
| 免疫原 |
CatNo: Ag24662 Product name: Recombinant human SLC25A29 protein Source: e coli.-derived, PET30a Tag: 6*His Domain: 1-146 aa of BC132966 Sequence: MGLTFINALVFGVQGNTLRALGHDSPLNQFLAGAAAGAIQCVICCPMELAKTRLQLQDAGPARTYKGSLDCLAQIYGHEGLRGVNRGMVSTLLRETPSFGVYFLTYDALTRALGCEPGDRLLVPKLLLAGGTSGIVSWLSTYPVDV 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | solute carrier family 25, member 29 |
| 别名 | C14orf69, CACT-like, Carnitine/acylcarnitine translocase-like, Mitochondrial basic amino acids transporter, Mitochondrial carnitine/acylcarnitine carrier protein CACL |
| 计算分子量 | 303 aa, 32 kDa |
| 观测分子量 | 32 kDa |
| GenBank蛋白编号 | BC132966 |
| 基因名称 | SLC25A29 |
| Gene ID (NCBI) | 123096 |
| RRID | AB_2880593 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q8N8R3 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SLC25A29 is a mitochondrial transporter belonging to the SLC25 superfamily, which facilitates the exchange of metabolites across the inner mitochondrial membrane. Initially reported as a carnitine/acylcarnitine-like carrier, it is now recognized as a key amino acid transporter, particularly for arginine and lysine, contributing to mitochondrial protein synthesis and amino acid degradation. SLC25A29 is primarily expressed in the heart, brain, liver, and kidneys and is associated with metabolic disorders and vascular diseases. Its expression is regulated by histone lactose modification and is associated with conditions such as lung adenocarcinoma and diabetic retinopathy.(PMID: 24652292; 37775731;29459713; 40244673)
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for SLC25A29 antibody 26663-1-AP | Download protocol |
| WB protocol for SLC25A29 antibody 26663-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
