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SLC25A18 Polyclonal antibody

SLC25A18 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, IP, WB, ELISA

Conjugate

Unconjugated

Cat No : 17348-1-AP

Print datasheet

Synonyms

GC 2, GC2, Glutamate/H(+) symporter 2, SLC25A18

验证数据展示

  • WB analysis using 17348-1-AP

    WB analysis using 17348-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17348-1-AP (SLC25A18 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse brain using 17348-1-AP

    IP experiment of mouse brain using 17348-1-AP

    IP result of anti-SLC25A18 (IP:17348-1-AP, 4ug; Detection:17348-1-AP 1:500) with mouse brain tissue lysate 4400ug.

  • IHC staining of mouse brain using 17348-1-AP

    IHC staining of mouse brain using 17348-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17348-1-AP (SLC25A18 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 17348-1-AP

    IHC staining of mouse brain using 17348-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17348-1-AP (SLC25A18 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inmouse liver tissue, mouse kidney tissue, mouse heart tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IHCSee 1 publications below

产品信息

17348-1-AP targets SLC25A18 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications IHC, IP, WB, ELISA
Cited ApplicationsIHC
Tested Reactivity human, mouse
Cited Reactivityhuman
Immunogen SLC25A18 fusion protein Ag11420 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name solute carrier family 25 (mitochondrial carrier), member 18
Synonyms GC 2, GC2, Glutamate/H(+) symporter 2, SLC25A18
Calculated Molecular Weight 315 aa, 34 kDa
Observed Molecular Weight 32-35 kDa
GenBank Accession NumberBC031644
Gene Symbol SLC25A18
Gene ID (NCBI) 83733
RRIDAB_2189889
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H1K4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SLC25A18, also named as GC 2, belongs to the mitochondrial transporter family (SLC25). It is located in inner mitochondrial membrane which involved in the transport of glutamate either with H+ or in exchange for OH-. SLC25A18 is highly expressed in brain, testis, lung, kidney, liver, spleen and skeletal muscle. 17348-1-AP antibody detects the band around 32-35 kDa in SDS-PAGE.   (PMID: 14598172)

实验方案

Product Specific Protocols
WB protocol for SLC25A18 antibody 17348-1-APDownload protocol
IHC protocol for SLC25A18 antibody 17348-1-APDownload protocol
IP protocol for SLC25A18 antibody 17348-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

Am J Cancer Res

SLC25A18 has prognostic value in colorectal cancer and represses Warburg effect and cell proliferation via Wnt signaling.

Authors - Li Liang
View Article