验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293T cells, A431 cells, HEK-293 cells, mouse kidney tissue, mouse liver tissue, Raji cells, mouse ovary tissue |
| Positive IP detected in | mouse kidney tissue |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | U2OS cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
10789-1-AP targets SLC25A13 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human |
| 免疫原 |
CatNo: Ag1242 Product name: Recombinant human SLC25A13 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 336-675 aa of BC006566 Sequence: SVAGAVGATAVYPIDLVKTRMQNQRSTGSFVGELMYKNSFDCFKKVLRYEGFFGLYRGLLPQLLGVAPEKAIKLTVNDFVRDKFMHKDGSVPLAAEILAGGCAGGSQVIFTNPLEIVKIRLQVAGEITTGPRVSALSVVRDLGFFGIYKGAKACFLRDIPFSAIYFPCYAHVKASFANEDGQVSPGSLLLAGAIAGMPAASLVTPADVIKTRLQVAARAGQTTYSGVIDCFRKILREEGPKALWKGAGARVFRSSPQFGVTLLTYELLQRWFYIDFGGVKPMGSEPVPKSRINLPAPNPDHVGGYKLAVATFAGIENKFGLYLPLFKPSVSTSKAIGGGP 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | solute carrier family 25, member 13 (citrin) |
| 别名 | Mitochondrial aspartate glutamate carrier 2, Electrogenic aspartate/glutamate antiporter SLC25A13, mitochondrial, Citrin, Calcium-binding mitochondrial carrier protein Aralar2, ARALAR-related gene 2 |
| 计算分子量 | 74 kDa |
| 观测分子量 | 60-63 kDa |
| GenBank蛋白编号 | BC006566 |
| 基因名称 | SLC25A13 |
| Gene ID (NCBI) | 10165 |
| RRID | AB_2189738 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9UJS0 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SLC25A13, also known as citrin, has been identified as a mitochondrial aspartate-glutamate carrier protein and may be implicated in metabolic compartmentation. It consists of 675 amino acid residues and harbors four EF-hands and six mitochondrial transmembranous (TM) spanners. Mutations in SLC25A13 gene cause adult-onset type II citrullinemia (CTLN2) and neonatal intrahepatic cholestasis (NICCD). The complete deletion or cleaved SLA25A13 proteins have been reported in patients with SLC25A13 mutation.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for SLC25A13 antibody 10789-1-AP | Download protocol |
| IHC protocol for SLC25A13 antibody 10789-1-AP | Download protocol |
| IP protocol for SLC25A13 antibody 10789-1-AP | Download protocol |
| WB protocol for SLC25A13 antibody 10789-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
