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验证数据展示

  • WB analysis of LNCaP using 67467-1-Ig (same clone as 67467-1-PBS)

    WB analysis of LNCaP using 67467-1-Ig (same clone as 67467-1-PBS)

    LNCaP cells were subjected to SDS PAGE followed by western blot with 67467-1-Ig (SLC25A12 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67467-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 67467-1-Ig (same clone as 67467-1-PBS)

    IHC staining of mouse brain using 67467-1-Ig (same clone as 67467-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67467-1-Ig (SLC25A12 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67467-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50650-2

    Cytometric bead array standard curve of MP50650-2

    Cytometric bead array standard curve of MP50650-2, SLC25A12 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67467-4-PBS. Detection antibody: 67467-1-PBS. Standard:Ag13576. Range: 1.563-100 ng/mL.

SLC25A12 Monoclonal antibody, PBS Only (Detector)

SLC25A12 Monoclonal Antibody for WB, IHC, Cytometric bead array, Indirect ELISA
Cat No. 67467-1-PBS

产品说明书

CloneNo. 1F2B10

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse

应用

WB, IHC, Cytometric bead array, Indirect ELISA

别名

AGC1, Araceli hiperlarga, Aralar, Aralar1, Electrogenic aspartate/glutamate antiporter SLC25A12, mitochondrial

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

67467-1-PBS targets SLC25A12 as part of a matched antibody pair:

MP50650-2: 67467-4-PBS capture and 67467-1-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原 SLC25A12 fusion protein Ag13576 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 solute carrier family 25 (mitochondrial carrier, Aralar), member 12
别名 AGC1, Araceli hiperlarga, Aralar, Aralar1, Electrogenic aspartate/glutamate antiporter SLC25A12, mitochondrial
计算分子量 75 kDa, 63 kDa
观测分子量 70-75 kDa
GenBank蛋白编号BC016932
基因名称 SLC25A12
Gene ID (NCBI) 8604
RRIDAB_2882696
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDO75746
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SLC25A12, also known as ARALAR1, is a key component of the malate-aspartate shuttle, a metabolic system that facilitates the transfer of reducing equivalents (NADH) from the cytoplasm into mitochondria, thereby supporting oxidative phosphorylation and ATP production. SLC25A12 dysfunction results in impaired mitochondrial energy production, which can lead to neuronal damage, developmental delays, and myelination defects (PMID: 20015484).