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验证数据展示

  • WB analysis using 12253-1-AP

    WB analysis using 12253-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12253-1-AP (SLC25A11 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IP experiment of HEK-293 using 12253-1-AP

    IP experiment of HEK-293 using 12253-1-AP

    IP result of anti-SLC25A11 (IP:12253-1-AP, 4ug; Detection:12253-1-AP 1:1000) with HEK-293 cells lysate 1560ug.

  • IF Staining of HEK-293 using 12253-1-AP

    IF Staining of HEK-293 using 12253-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using 12253-1-AP (SLC25A11 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IHC staining of human stomach cancer using 12253-1-AP

    IHC staining of human stomach cancer using 12253-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 12253-1-AP (SLC25A11 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Featured Product
  • KD/KO验证

SLC25A11 Polyclonal antibody

SLC25A11 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 12253-1-AP

产品说明书

引用文献(3)

宿主/亚型

Rabbit / IgG

种属反应性

human and More (1)

应用

WB, IHC, IF/ICC, IP, ELISA and More (1)

别名

SLC20A4, OGCP, OGC, Mitochondrial 2-oxoglutarate/malate carrier protein, M2OM

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inJurkat cells, human kidney tissue, HEK-293 cells, Raji cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHEK-293 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12253-1-AP targets SLC25A11 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
文献引用应用WB, IF, IP
经测试反应性 human
文献引用反应性human, mouse
免疫原 SLC25A11 fusion protein Ag2903 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 solute carrier family 25 (mitochondrial carrier; oxoglutarate carrier), member 11
别名 SLC20A4, OGCP, OGC, Mitochondrial 2-oxoglutarate/malate carrier protein, M2OM
计算分子量 314 aa, 34 kDa
观测分子量 28-34 kDa
GenBank蛋白编号BC016294
基因名称 SLC25A11
Gene ID (NCBI) 8402
RRIDAB_2877840
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ02978
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for SLC25A11 antibody 12253-1-APDownload protocol
IHC protocol for SLC25A11 antibody 12253-1-APDownload protocol
IF protocol for SLC25A11 antibody 12253-1-APDownload protocol
IP protocol for SLC25A11 antibody 12253-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IF,IP

EMBO Rep

LINC00493-encoded microprotein SMIM26 exerts anti-metastatic activity in renal cell carcinoma

Authors - Kun Meng
  • KD Validated
View Article
human,mouseWB,IF

Proc Natl Acad Sci U S A

Mitochondrial outer membrane protein FUNDC2 promotes ferroptosis and contributes to doxorubicin-induced cardiomyopathy

Authors - Na Ta
  • KO Validated
View Article
humanWB

Science

Autoregulatory control of mitochondrial glutathione homeostasis

Authors - Yuyang Liu
View Article