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SLC25A11 Polyclonal antibody

SLC25A11 Polyclonal Antibody for IF/ICC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

IF/ICC, IP, WB,ELISA

Conjugate

Unconjugated

Cat No : 12253-1-AP

Print datasheet

Synonyms

OGC, OGCP, SLC20A4, SLC25A11

验证数据展示

  • WB analysis using 12253-1-AP

    WB analysis using 12253-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12253-1-AP (SLC25A11 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IP experiment of HEK-293 using 12253-1-AP

    IP experiment of HEK-293 using 12253-1-AP

    IP result of anti-SLC25A11 (IP:12253-1-AP, 4ug; Detection:12253-1-AP 1:1000) with HEK-293 cells lysate 1560ug.

  • IF Staining of HEK-293 using 12253-1-AP

    IF Staining of HEK-293 using 12253-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using 12253-1-AP (SLC25A11 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inJurkat cells, human kidney tissue, HEK-293 cells, Raji cells
Positive IP detected inHEK-293 cells
Positive IF detected inHEK-293 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12253-1-AP targets SLC25A11 in WB, IP, IF, ELISA applications and shows reactivity with human samples.

Tested Applications IF/ICC, IP, WB,ELISA
Cited ApplicationsWB, IF
Tested Reactivity human
Cited Reactivityhuman, mouse
Immunogen SLC25A11 fusion protein Ag2903 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name solute carrier family 25 (mitochondrial carrier; oxoglutarate carrier), member 11
Synonyms OGC, OGCP, SLC20A4, SLC25A11
Calculated Molecular Weight 314 aa, 34 kDa
Observed Molecular Weight 28-34 kDa
GenBank Accession NumberBC016294
Gene Symbol SLC25A11
Gene ID (NCBI) 8402
RRIDAB_2877840
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ02978
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for SLC25A11 antibody 12253-1-APDownload protocol
IF protocol for SLC25A11 antibody 12253-1-APDownload protocol
IP protocol for SLC25A11 antibody 12253-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
human,mouseWB,IF

Proc Natl Acad Sci U S A

Mitochondrial outer membrane protein FUNDC2 promotes ferroptosis and contributes to doxorubicin-induced cardiomyopathy

Authors - Na Ta
  • KO Validated
View Article
humanWB

Science

Autoregulatory control of mitochondrial glutathione homeostasis

Authors - Yuyang Liu
View Article