- Featured Product
- KD/KO Validated
SLC25A10 Polyclonal antibody
SLC25A10 Polyclonal Antibody for IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
IHC, IP, WB, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, A549 cells, mouse liver tissue, mouse kidney tissue, HepG2 cells, PC-3 cells |
| Positive IP detected in | mouse liver tissue |
| Positive IHC detected in | human lung tissue, mouse kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 2 publications below |
| IHC | See 2 publications below |
产品信息
12086-1-AP targets SLC25A10 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | IHC, IP, WB, ELISA |
| Cited Applications | WB, IHC |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Immunogen | SLC25A10 fusion protein Ag2719 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | solute carrier family 25 (mitochondrial carrier; dicarboxylate transporter), member 10 |
| Synonyms | DIC, SLC25A10 |
| Calculated Molecular Weight | 296 aa, 32 kDa |
| Observed Molecular Weight | 29-31 kDa |
| GenBank Accession Number | BC015797 |
| Gene Symbol | SLC25A10 |
| Gene ID (NCBI) | 1468 |
| RRID | AB_10859816 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9UBX3 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SLC25A10, also known as dicarboxylate ion carrier (DIC), is a mitochondrial inner membrane carrier protein which catalyzes the transport of dicarboxylates such as malate and succinate across the mitochondrial membrane in exchange for phosphate, sulfate, and thiosulfate. SLC25A10 is expressed at highest levels in kidney and liver, lower levels in lung, spleen, heart, and brain, and lowest levels in skeletal muscle. Two isoforms of SLC25A10 exist due to the alternative splicing.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for SLC25A10 antibody 12086-1-AP | Download protocol |
| IHC protocol for SLC25A10 antibody 12086-1-AP | Download protocol |
| IP protocol for SLC25A10 antibody 12086-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Hum Cell The role of PYCR1 in inhibiting 5-fluorouracil-induced ferroptosis and apoptosis through SLC25A10 in colorectal cancer | ||
