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验证数据展示

  • WB analysis using 66771-1-Ig (same clone as 66771-1-PBS)

    WB analysis using 66771-1-Ig (same clone as 66771-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66771-1-Ig (SLC25A1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 66771-1-PBS in a different storage buffer formulation.

  • WB analysis using 66771-1-Ig (same clone as 66771-1-PBS)

    WB analysis using 66771-1-Ig (same clone as 66771-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66771-1-Ig (SLC25A1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66771-1-PBS in a different storage buffer formulation.

SLC25A1 Monoclonal antibody, PBS Only

SLC25A1 Monoclonal Antibody for WB, Indirect ELISA
Cat No. 66771-1-PBS

产品说明书

CloneNo. 1F11A3

宿主/亚型

Mouse / IgG3

种属反应性

Human, Mouse, Rat

应用

WB, Indirect ELISA

别名

Citrate transport protein, CTP, SLC20A3, SLC25A1, Tricarboxylate carrier protein

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

66771-1-PBS targets SLC25A1 in WB, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat samples.

经测试应用 WB, Indirect ELISA Application Description
经测试反应性 Human, Mouse, Rat
免疫原

CatNo: Ag7520

Product name: Recombinant human SLC25A1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-311 aa of BC004980

Sequence: MPAPRAPRALAAAAPASGKAKLTHPGKAILAGGLAGGIEICITFPTEYVKTQLQLDERSHPPRYRGIGDCVRQTVRSHGVLGLYRGLSSLLYGSIPKAAVRFGMFEFLSNHMRDAQGRLDSTRGLLCGLGAGVAEAVVVVCPMETIKVKFIHDQTSPNPKYRGFFHGVREIVREQGLKGTYQGLTATVLKQGSNQAIRFFVMTSLRNWYRGDNPNKPMNPLITGVFGAIAGAASVFGNTPLDVIKTRMQGLEAHKYRNTWDCGLQILKKEGLKAFYKGTVPRLGRVCLDVAIVFVIYDEVVKLLNKVWKTD

种属同源性预测
宿主/亚型 Mouse / IgG3
抗体类别 Monoclonal
产品类型 Antibody
全称 solute carrier family 25 (mitochondrial carrier; citrate transporter), member 1
别名 Citrate transport protein, CTP, SLC20A3, SLC25A1, Tricarboxylate carrier protein
计算分子量 34 kDa
观测分子量 34 kDa
GenBank蛋白编号BC004980
基因名称 SLC25A1
Gene ID (NCBI) 6576
RRIDAB_2882117
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP53007
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SLC25A1, also known as CIC or CTP, is a mitochondrial citrate transporter that exports citrate from the mitochondria to the cytosol. SLC25A1 is highly expressed in several tumor types. Recently it has been identified as a novel transcriptional target of mutant p53 and a negative tumor prognostic marker. In addition, defects in SLC25A1 has been found as a cause of combined D-2- and L-2-hydroxyglutaric aciduria. This antibody specifically recognized the endogenous SLC25A1; no protein was detected in cells from subjects containing two alleles with truncating mutations with this antibody. (24681808, 23561848)

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