验证数据展示
经过测试的应用
| Positive WB detected in | mouse eye tissue, A375 cells, rat eye tissue |
| Positive IHC detected in | human thyroid cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| IHC | See 2 publications below |
产品信息
27747-1-AP targets SLC24A5 in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | IHC |
| 经测试反应性 | Human, mouse, rat |
| 文献引用反应性 | mouse |
| 免疫原 |
CatNo: Ag24254 Product name: Recombinant human SLC24A5 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 412-500 aa of BC113628 Sequence: LCLGIPWFIKTAFINGSAPAEVNSRGLTYITISLNISIIFLFLAVHFNGWKLDRKLGIVCLLSYLGLATLSVLYELGIIGNNKIRGCGG 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | solute carrier family 24, member 5 |
| 别名 | JSX, NCKX5, SHEP4, SLC24A5 |
| 计算分子量 | 500 aa, 55 kDa |
| 观测分子量 | 45-50 kDa |
| GenBank蛋白编号 | BC113628 |
| 基因名称 | SLC24A5 |
| Gene ID (NCBI) | 283652 |
| RRID | AB_2880959 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q71RS6 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for SLC24A5 antibody 27747-1-AP | Download protocol |
| IHC protocol for SLC24A5 antibody 27747-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Lab Invest Nuclear receptor binding factor 2 (NRBF2) is required for learning and memory. | ||
Pigment Cell Melanoma Res Impact of a SLC24A5 variant on the retinal pigment epithelium of a Japanese patient with oculocutaneous albinism type 6.
|