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验证数据展示

  • WB analysis using 27747-1-AP

    WB analysis using 27747-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27747-1-AP (SLC24A5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human thyroid cancer using 27747-1-AP

    IHC staining of human thyroid cancer using 27747-1-AP

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 27747-1-AP (SLC24A5 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human thyroid cancer using 27747-1-AP

    IHC staining of human thyroid cancer using 27747-1-AP

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 27747-1-AP (SLC24A5 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis of A375 using 27747-1-AP

    WB analysis of A375 using 27747-1-AP

    A375 cells were subjected to SDS PAGE followed by western blot with 27747-1-AP (SLC24A5 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Featured Product
  • KD/KO验证

SLC24A5 Polyclonal antibody

SLC24A5 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 27747-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

Human, mouse, rat

应用

WB, IHC, ELISA

别名

JSX, NCKX5, SHEP4, SLC24A5

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse eye tissue, A375 cells, rat eye tissue
Positive IHC detected inhuman thyroid cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

KD/KOSee 1 publications below
IHCSee 2 publications below

产品信息

27747-1-AP targets SLC24A5 in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用IHC
经测试反应性 Human, mouse, rat
文献引用反应性mouse
免疫原 SLC24A5 fusion protein Ag24254 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 solute carrier family 24, member 5
别名 JSX, NCKX5, SHEP4, SLC24A5
计算分子量 500 aa, 55 kDa
观测分子量 45-50 kDa
GenBank蛋白编号BC113628
基因名称 SLC24A5
Gene ID (NCBI) 283652
RRIDAB_2880959
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ71RS6
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for SLC24A5 antibody 27747-1-APDownload protocol
IHC protocol for SLC24A5 antibody 27747-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIHC

Lab Invest

Nuclear receptor binding factor 2 (NRBF2) is required for learning and memory.

Authors - Xiaosen Ouyang
View Article
mouseIHC

Pigment Cell Melanoma Res

Impact of a SLC24A5 variant on the retinal pigment epithelium of a Japanese patient with oculocutaneous albinism type 6.

Authors - Toru Saito
  • KO Validated
View Article