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  • Featured Product
  • KD/KO Validated

SLC24A4 Polyclonal antibody

SLC24A4 Polyclonal Antibody for IF-P, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

IF-P, IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 18992-1-AP

Print datasheet

Synonyms

NCKX4, SHEP6, SLC24A2, SLC24A4

验证数据展示

  • WB analysis using 18992-1-AP

    WB analysis using 18992-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 18992-1-AP (SLC24A4 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa cells using 18992-1-AP

    WB analysis of HeLa cells using 18992-1-AP

    WB result of SLC24A4 antibody (18992-1-AP, 1:1000) with si-Control and si-SLC24A4 transfected HeLa cells.

  • IF Staining of mouse eye using 18992-1-AP

    IF Staining of mouse eye using 18992-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse eye tissue using SLC24A4 antibody (18992-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IF Staining of mouse eye using 18992-1-AP

    IF Staining of mouse eye using 18992-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse eye tissue using SLC24A4 antibody (18992-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IHC staining of mouse brain using 18992-1-AP

    IHC staining of mouse brain using 18992-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18992-1-AP (SLC24A4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 18992-1-AP

    IHC staining of mouse brain using 18992-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18992-1-AP (SLC24A4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 18992-1-AP

    IHC staining of mouse brain using 18992-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18992-1-AP (SLC24A4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 18992-1-AP

    IHC staining of mouse brain using 18992-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18992-1-AP (SLC24A4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inmouse brain tissue, HeLa cells, mouse heart tissue
Positive IHC detected inmouse brain tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse eye tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

18992-1-AP targets SLC24A4 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications IF-P, IHC, WB, ELISA
Cited ApplicationsWB, IHC
Tested Reactivity human, mouse
Cited Reactivitymouse, rat
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name solute carrier family 24 (sodium/potassium/calcium exchanger), member 4
Synonyms NCKX4, SHEP6, SLC24A2, SLC24A4
Calculated Molecular Weight 69 kDa
Observed Molecular Weight 62-90 kDa
GenBank Accession NumberNM_153646
Gene Symbol SLC24A4
Gene ID (NCBI) 123041
RRIDAB_10596939
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8NFF2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SLC24A4, also named as NCKX4, belongs to the sodium/potassium/calcium exchanger family. SLC24A4 transports 1 Ca2+ and 1 K+ in exchange for 4 Na+. Genetic variations in SLC24A4 are associated with skin/hair/eye pigmentation type 6 (SHEP6).

实验方案

Product Specific Protocols
WB protocol for SLC24A4 antibody 18992-1-APDownload protocol
IHC protocol for SLC24A4 antibody 18992-1-APDownload protocol
IF protocol for SLC24A4 antibody 18992-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIHC

Bone

Deletion of epithelial cell-specific p130Cas impairs the maturation stage of amelogenesis.

Authors - Akane Inoue
View Article
ratWB

J Cell Physiol

Identification of novel candidate genes involved in mineralization of dental enamel by genome-wide transcript profiling.

Authors - Lacruz Rodrigo S RS
View Article
mouseWB

Calcif Tissue Int

Reduced Protein Expression of the Na(+)/Ca(2+)+K(+)-Exchanger (SLC24A4) in Apical Plasma Membranes of Maturation Ameloblasts of Fluorotic Mice.

Authors - A L J J Bronckers
View Article
mouseWB

Cells Tissues Organs

Expression of the sodium/calcium/potassium exchanger, NCKX4, in ameloblasts.

Authors - Hu Ping P
View Article