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SLC22A7 Monoclonal antibody

SLC22A7 Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat

Applications

IF, IHC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

3D3C3

Cat No : 67479-1-Ig

Print datasheet

Synonyms

hOAT2, NLT, Novel liver transporter, OAT2, Organic anion transporter 2, SLC22A7

验证数据展示

  • WB analysis of SMMC-7721 using 67479-1-Ig

    WB analysis of SMMC-7721 using 67479-1-Ig

    SMMC-7721 cells were subjected to SDS PAGE followed by western blot with 67479-1-Ig (SLC22A7 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 67479-1-Ig

    IHC staining of human liver cancer using 67479-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67479-1-Ig (SLC22A7 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SLC22A7 antibody included
  • IF Staining of HEK-293 using 67479-1-Ig

    IF Staining of HEK-293 using 67479-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using SLC22A7 antibody (67479-1-Ig, Clone: 3D3C3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • IHC staining of human liver using 67479-1-Ig

    IHC staining of human liver using 67479-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67479-1-Ig (SLC22A7 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SLC22A7 antibody included
  • IHC staining of human liver cancer using 67479-1-Ig

    IHC staining of human liver cancer using 67479-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67479-1-Ig (SLC22A7 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SLC22A7 antibody included
  • IHC staining of mouse liver using 67479-1-Ig

    IHC staining of mouse liver using 67479-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 67479-1-Ig (SLC22A7 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SLC22A7 antibody included

经过测试的应用

Positive WB detected inSMMC-7721 cells
Positive IHC detected inhuman liver tissue, human liver cancer tissue, mouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHEK-293 cells
Planning an IHC experiment? We recommend our IHCeasy SLC22A7 Ready-To-Use IHC Kit. SLC22A7 primary antibody included.
Planning an IF experiment? We recommend our CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:3000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67479-1-Ig targets SLC22A7 in WB, IF, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications IF, IHC, WB, ELISA
Tested Reactivity Human, mouse, rat
Immunogen SLC22A7 fusion protein Ag25234 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name solute carrier family 22 (organic anion transporter), member 7
Synonyms hOAT2, NLT, Novel liver transporter, OAT2, Organic anion transporter 2, SLC22A7
Calculated Molecular Weight 60 kDa
Observed Molecular Weight 60 kDa
GenBank Accession NumberBC033805
Gene Symbol SLC22A7
Gene ID (NCBI) 10864
RRIDAB_2882706
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9Y694
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Organic anion transporter (OAT)2 (SLC22A7) belongs to the solute carrier group of membrane transport proteins that mediate cellular uptake of numerous organic ions including xenobiotics and endogenous substrates (PMID: 9529348, 20190416). SLC22A7 was originally identified as a novel liver-specific transporter because of its predominant mRNA expression in the rat liver (PMID: 15900017, 25904762). Human SLC22A7 exhibits a robust transport function for a wide array of naturally occurring nucleobases, nucleosides, and nucleotides with a particular role for cGMP (PMID: 18216183).

实验方案

Product Specific Protocols
WB protocol for SLC22A7 antibody 67479-1-IgDownload protocol
IHC protocol for SLC22A7 antibody 67479-1-IgDownload protocol
IF protocol for SLC22A7 antibody 67479-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols