验证数据展示
经过测试的应用
Positive WB detected in | MCF-7 cells, PC-3 cells |
Positive IHC detected in | human breast cancer tissue, human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | THP-1 cells |
推荐稀释比
应用 | 推荐稀释比 |
---|---|
Western Blot (WB) | WB : 1:1000-1:4000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
26833-1-AP targets SLC22A16 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
经测试反应性 | human |
免疫原 | SLC22A16 fusion protein Ag25270 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | solute carrier family 22 (organic cation/carnitine transporter), member 16 |
别名 | Carnitine transporter 2, CT2, Flipt 2, FLIPT2, OCT6, OKB1, SLC22A16 |
计算分子量 | 65 kDa |
观测分子量 | 68 kDa |
GenBank蛋白编号 | BC047565 |
基因名称 | SLC22A16 |
Gene ID (NCBI) | 85413 |
RRID | AB_2923576 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | Q86VW1 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SLC22A16, also named as CT2, FLIPT2, or OCT6, belongs to the major facilitator (TC 2.A.1) superfamily and organic cation transporter (TC 2.A.1.19) family. SLC22A16 encodes an organic zwitterion transporter protein that transports carnitine and some anticancer drugs, including anthracyclines. As a carnitine transporter, SLC22A16 is involved in the regulation of fatty acid oxidation (FAO), which plays an important role also in the metabolic transformation of cancer cells. SLC22A16 expression in cancer cells is associated with increased sensitivity to the cytotoxic effects of doxorubicin. SLC22A16 has 3 isoforms with the molecular mass of 58, 61 and 65 kDa (PMID: 35761855, 35608746).
实验方案
Product Specific Protocols | |
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WB protocol for SLC22A16 antibody 26833-1-AP | Download protocol |
IHC protocol for SLC22A16 antibody 26833-1-AP | Download protocol |
IF protocol for SLC22A16 antibody 26833-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |