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验证数据展示

  • WB analysis using 31069-1-AP

    WB analysis using 31069-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31069-1-AP (SLC22A10 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse liver using 31069-1-AP

    IHC staining of mouse liver using 31069-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 31069-1-AP (SLC22A10 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse liver using 31069-1-AP

    IHC staining of mouse liver using 31069-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 31069-1-AP (SLC22A10 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

SLC22A10 Polyclonal antibody

SLC22A10 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 31069-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Human, mouse, rat

应用

WB, IHC, ELISA

别名

hOAT5, OAT5, Organic anion transporter 5, SLC22A10

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHEK-293T cells, PC-12 cells, mouse kidney tissue, mouse liver tissue, rat kidney tissue, rat liver tissue
Positive IHC detected inmouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31069-1-AP targets SLC22A10 in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 Human, mouse, rat
免疫原

CatNo: Ag34521

Product name: Recombinant human SLC22A10 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 281-349 aa of NM_001039752

Sequence: ESARWLIITNKLDEGLKALRKVARTNGIKNAEETLNIEVVRSTMQEELDAAQTKTTVCDLFRNPSMRKR

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 solute carrier family 22, member 10
别名 hOAT5, OAT5, Organic anion transporter 5, SLC22A10
计算分子量541 aa, 60 kDa
观测分子量70 kDa
GenBank蛋白编号NM_001039752
基因名称 SLC22A10
Gene ID (NCBI) 387775
RRIDAB_3669842
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ63ZE4
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Organic anion transporter 5 (OAT5), also known as SLC22A10, belongs to the solute carrier group of membrane transport proteins that mediate cellular uptake of endogenous and exogenous organic ions (PMID: 20865662). Rare variants in SLC22A10 may be associated with Alzheimer's disease CSF profile of neuronal injury and inflammation (PMID: 35173266).

实验方案

Product Specific Protocols
IHC protocol for SLC22A10 antibody 31069-1-APDownload protocol
WB protocol for SLC22A10 antibody 31069-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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