验证数据展示
经过测试的应用
| Positive WB detected in | mouse liver tissue, SH-SY5Y cells, mouse skeletal muscle tissue, mouse kidney tissue, human placenta tissue, rat heart tissue, 3T3-L1 cells, rat liver tissue |
| Positive IP detected in | mouse liver tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
13407-1-AP targets SLC19A3 in WB, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IP, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag4053 Product name: Recombinant human SLC19A3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 188-315 aa of BC032014 Sequence: LFLPMPKKSMFFHAKPSREIKKSSSVNPVLEETHEGEAPGCEEQKPTSEILSTSGKLNKGQLNSLKPSNVTVDVFVQWFQDLKECYSSKRLFYWSLWWAFATAGFNQVLNYVQILWDYKAPSQDSSIY 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | solute carrier family 19, member 3 |
| 别名 | SLC19A3, Thiamine transporter 2, ThTr 2, THTR2 |
| 计算分子量 | 496 aa, 56 kDa |
| 观测分子量 | 63-70 kDa |
| GenBank蛋白编号 | BC032014 |
| 基因名称 | SLC19A3 |
| Gene ID (NCBI) | 80704 |
| RRID | AB_2187997 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9BZV2 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IP protocol for SLC19A3 antibody 13407-1-AP | Download protocol |
| WB protocol for SLC19A3 antibody 13407-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Sci Transl Med CPEB alteration and aberrant transcriptome-polyadenylation lead to a treatable SLC19A3 deficiency in Huntington's disease. | ||
Nat Commun Enterotoxigenic Escherichia coli heat-labile toxin drives enteropathic changes in small intestinal epithelia | ||
Front Cell Dev Biol Impact of Metformin Treatment on Human Placental Energy Production and Oxidative Stress. | ||
J Biol Chem Hypoxia inhibits colonic uptake of the microbiota-generated forms of vitamin B1 via HIF-1α-mediated transcriptional regulation of their transporters. | ||
Front Pharmacol Chronic Voluntary Alcohol Drinking Causes Anxiety-like Behavior, Thiamine Deficiency, and Brain Damage of Female Crossed High Alcohol Preferring Mice. |
