验证数据展示
经过测试的应用
| Positive WB detected in | mouse brain tissue, human placenta tissue, mouse kidney tissue, mouse skeletal muscle tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:12000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 4 publications below |
产品信息
20751-1-AP targets NPT1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | NPT1 fusion protein Ag12819 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | solute carrier family 17 (sodium phosphate), member 1 |
| 别名 | SLC17A1, Na/Pi-4, NAPI 1, Renal Na(+)-dependent phosphate cotransporter 1, Renal sodium-dependent phosphate transport protein 1 |
| 计算分子量 | 467 aa, 51 kDa |
| 观测分子量 | 50 kDa, 60-64 kDa |
| GenBank蛋白编号 | BC101745 |
| 基因名称 | NPT1 |
| Gene ID (NCBI) | 6568 |
| RRID | AB_10693677 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q14916 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
NPT1 (also known as SLC17A1) is a member of sodium-dependent inorganic phosphate transporters (NPT), which regulates entrance into the cellular membrane. NPT1 is mainly expressed in the kidney transporting small organic anions such as PAH (para-aminohippurate), but it is also found in the liver and brain. NTP1 localizes to the apical membrane of renal proximal tubular cells. NPT1 exits two isoforms due to the alternative splicing. Western blot analysis using this antibody detected a major band around 45-55 kDa in kidney tissue.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for NPT1 antibody 20751-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Exp Mol Med Impaired Na+-K+-ATPase signaling in renal proximal tubule contributes to hyperuricemia-induced renal tubular injury. | ||
Phytomedicine Eurycomanol alleviates hyperuricemia by promoting uric acid excretion and reducing purine synthesis. | ||
Am J Physiol Renal Physiol Transcriptomics of SGLT2-positive early proximal tubule segments in mice: response to type 1 diabetes, SGLT1/2 inhibition, or GLP1 receptor agonism | ||
Front Pharmacol Effect of Eurycoma longifolia Stem Extract on Uric Acid Excretion in Hyperuricemia Mice. |
