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SLC16A4 Polyclonal antibody

SLC16A4 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 20889-1-AP

Print datasheet

Synonyms

MCT 4, MCT 5, MCT4, MCT5, Monocarboxylate transporter 4, Monocarboxylate transporter 5, SLC16A4

验证数据展示

  • WB analysis of Jurkat using 20889-1-AP

    WB analysis of Jurkat using 20889-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 20889-1-AP (SLC16A4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human stomach cancer using 20889-1-AP

    IHC staining of human stomach cancer using 20889-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 20889-1-AP (SLC16A4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis using 20889-1-AP

    WB analysis using 20889-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 20889-1-AP (SLC16A4 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inJurkat cells
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

20889-1-AP targets SLC16A4 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications IHC, WB, ELISA
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivityhuman, mouse
Immunogen SLC16A4 fusion protein Ag14190 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name solute carrier family 16, member 4 (monocarboxylic acid transporter 5)
Synonyms MCT 4, MCT 5, MCT4, MCT5, Monocarboxylate transporter 4, Monocarboxylate transporter 5, SLC16A4
Calculated Molecular Weight 487 aa, 54 kDa
Observed Molecular Weight 50 kDa
GenBank Accession NumberBC021664
Gene Symbol SLC16A4
Gene ID (NCBI) 9122
RRIDAB_2878757
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO15374
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SLC16A4, also named as MCT5, is a proton-linked monocarboxylate transporter which can catalyze the rapid transport across the plasma membrane of many monocarboxylates. It has been reported that SLC16A4 is expressed in human intestine, liver and kidney. (PMID:18996437)

实验方案

Product Specific Protocols
WB protocol for SLC16A4 antibody 20889-1-APDownload protocol
IHC protocol for SLC16A4 antibody 20889-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Arq Bras Cardiol

SLC26A4-AS1 Aggravates AngII-induced Cardiac Hypertrophy by Enhancing SLC26A4 Expression

Authors - Xiaoliang Han
View Article
humanWB

Int J Biochem Cell Biol

The CDK4/6 inhibitor PD0332991 stabilizes FBP1 by repressing MAGED1 expression in pancreatic ductal adenocarcinoma.

Authors - Bin Zhang
View Article