验证数据展示
经过测试的应用
| Positive WB detected in | Jurkat cells |
| Positive IHC detected in | human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 2 publications below |
产品信息
20889-1-AP targets SLC16A4 in WB, IHC, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse |
| 免疫原 | SLC16A4 fusion protein Ag14190 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | solute carrier family 16, member 4 (monocarboxylic acid transporter 5) |
| 别名 | MCT 4, MCT 5, MCT4, MCT5, Monocarboxylate transporter 4, Monocarboxylate transporter 5, SLC16A4 |
| 计算分子量 | 487 aa, 54 kDa |
| 观测分子量 | 50 kDa |
| GenBank蛋白编号 | BC021664 |
| 基因名称 | SLC16A4 |
| Gene ID (NCBI) | 9122 |
| RRID | AB_2878757 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O15374 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SLC16A4, also named as MCT5, is a proton-linked monocarboxylate transporter which can catalyze the rapid transport across the plasma membrane of many monocarboxylates. It has been reported that SLC16A4 is expressed in human intestine, liver and kidney. (PMID:18996437)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for SLC16A4 antibody 20889-1-AP | Download protocol |
| IHC protocol for SLC16A4 antibody 20889-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Arq Bras Cardiol SLC26A4-AS1 Aggravates AngII-induced Cardiac Hypertrophy by Enhancing SLC26A4 Expression | ||
Int J Biochem Cell Biol The CDK4/6 inhibitor PD0332991 stabilizes FBP1 by repressing MAGED1 expression in pancreatic ductal adenocarcinoma. |
