验证数据展示
经过测试的应用
| Positive IHC detected in | human kidney tissue, mouse kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 1 publications below |
| IHC | See 3 publications below |
产品信息
21722-1-AP targets SLC13A2 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag16379 Product name: Recombinant human SLC13A2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 129-231 aa of BC096277 Sequence: MLVTAFLSMWISNTATSAMMVPIAHAVLDQLHSSQASSNVEEGSNNPTFELQEPSPQKEVTKLDNGQALPVTSASSEGRAHLSQKHLHLTQCMSLCVCYSASI 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | solute carrier family 13 (sodium-dependent dicarboxylate transporter), member 2 |
| 别名 | SDCT1, NaDC-1, NADC1, NaDC 1, NaCT |
| 计算分子量 | 592 aa, 64 kDa |
| GenBank蛋白编号 | BC096277 |
| 基因名称 | SLC13A2 |
| Gene ID (NCBI) | 9058 |
| RRID | AB_2878912 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q13183 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SLC13A2 (Solute Carrier Family 13 Member 2) is involved in the transport of citric acid cycle intermediates, such as succinate, citrate, fumarate, and alpha-ketoglutarate (2-oxoglutarate), across cell membranes. In the liver, SLC13A2 has been shown to promote metabolic remodeling by transporting citrate, which is then converted to acetyl-CoA, a precursor for cholesterol synthesis and fatty acid metabolism (PMID: 39824985).
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for SLC13A2 antibody 21722-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Am J Physiol Renal Physiol Expression of sodium-dependent dicarboxylate transporter 1 (NaDC1/SLC13A2) in normal and neoplastic human kidney. | ||
Cancer Med Transcriptome analysis in primary colorectal cancer tissues from patients with and without liver metastases using next-generation sequencing. | ||
Am J Physiol Renal Physiol Regulation of Renal NaDC1 Expression and Citrate Excretion by NBCe1-A. | ||
Physiol Rep Effect of NBCe1 deletion on renal citrate and 2-oxoglutarate handling.
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