KCC2/SLC12A5-Specific Polyclonal antibody
KCC2/SLC12A5-Specific Polyclonal Antibody for IF-P, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
IF-P, IHC, WB, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | human brain tissue, mouse brain tissue, rat brain tissue |
Positive IHC detected in | human brain tissue, rat brain tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | mouse cerebellum tissue |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
Immunofluorescence (IF) | IF : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
19565-1-AP targets KCC2/SLC12A5-Specific in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | IF-P, IHC, WB, ELISA |
Cited Applications | WB, IF, IHC |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat |
Immunogen | Peptide 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | solute carrier family 12 (potassium-chloride transporter), member 5 |
Synonyms | hKCC2, K Cl cotransporter 2, KCC2, KCC2/SLC12A5-Specific, KIAA1176, Neuronal K Cl cotransporter, SLC12A5 |
Calculated Molecular Weight | 126 kDa |
Observed Molecular Weight | 130-150 kDa |
GenBank Accession Number | NM_020708 |
Gene Symbol | SLC12A5 |
Gene ID (NCBI) | 57468 |
RRID | AB_10638485 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q9H2X9 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for KCC2/SLC12A5-Specific antibody 19565-1-AP | Download protocol |
IHC protocol for KCC2/SLC12A5-Specific antibody 19565-1-AP | Download protocol |
IF protocol for KCC2/SLC12A5-Specific antibody 19565-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Neurobiol Dis HIV and opiates dysregulate K+- Cl- cotransporter 2 (KCC2) to cause GABAergic dysfunction in primary human neurons and Tat-transgenic mice. | ||
iScience Time-restricted feeding entrains long-term behavioral changes through the IGF2-KCC2 pathway. | ||
ASN Neuro Restoration of KCC2 Membrane Localization in Striatal Dopamine D2 Receptor-Expressing Medium Spiny Neurons Rescues Locomotor Deficits in HIV Tat-Transgenic Mice. | ||
Brain Behav Involvement of the BDNF-TrkB-KCC2 pathway in neuropathic pain after brachial plexus avulsion. | ||
Front Mol Neurosci Partially brain effects of injection of human umbilical cord mesenchymal stem cells at injury sites in a mouse model of thoracic spinal cord contusion |