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验证数据展示

  • IHC staining of human liver cancer using 15927-1-AP (same clone as 15927-1-PBS)

    IHC staining of human liver cancer using 15927-1-AP (same clone as 15927-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15927-1-AP (SLC12A4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15927-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 15927-1-AP (same clone as 15927-1-PBS)

    WB analysis of HeLa using 15927-1-AP (same clone as 15927-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 15927-1-AP (SLC12A4 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15927-1-PBS in a different storage buffer formulation.

  • IP experiment of HeLa using 15927-1-AP (same clone as 15927-1-PBS)

    IP experiment of HeLa using 15927-1-AP (same clone as 15927-1-PBS)

    IP result of anti-SLC12A4 (IP:15927-1-AP, 4ug; Detection:15927-1-AP 1:500) with HeLa cells lysate 3200ug. This data was developed using the same antibody clone with 15927-1-PBS in a different storage buffer formulation.

SLC12A4/KCC1 Polyclonal antibody, PBS Only

SLC12A4/KCC1 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 15927-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IP, Indirect ELISA

别名

SLC12A4, Electroneutral potassium-chloride cotransporter 1, Erythroid K-Cl cotransporter 1, KCC1

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

15927-1-PBS targets SLC12A4/KCC1 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag8762

Product name: Recombinant human SLC12A4 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 891-1085 aa of BC021193

Sequence: MKKDLAVFLYHLRLEAEVEVVEMHNSDISAYTYERTLMMEQRSQMLRQMRLTKTEREREAQLVKDRHSALRLESLYSDEEDESAVGADKIQMTWTRDKYMTETWDPSHAPDNFRELVHIKPDQSNVRRMHTAVKLNEVIVTRSHDARLVLLNMPGPPRNSEGDENYMEFLEVLTEGLERVLLVRGGGREVITIYS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 solute carrier family 12 (potassium/chloride transporters), member 4
别名 SLC12A4, Electroneutral potassium-chloride cotransporter 1, Erythroid K-Cl cotransporter 1, KCC1
计算分子量 1085 aa, 121 kDa
观测分子量 121 kDa
GenBank蛋白编号BC021193
基因名称 SLC12A4
Gene ID (NCBI) 6560
RRIDAB_2188666
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9UP95
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C
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