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验证数据展示

  • WB analysis using 55442-1-AP

    WB analysis using 55442-1-AP

    37°C incubated mouse kidney tissue were subjected to SDS PAGE followed by western blot with 55442-1-AP (SLC12A3/NCCT antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse kidney using 55442-1-AP

    IHC staining of mouse kidney using 55442-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 55442-1-AP (SLC12A3/NCCT antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse kidney using 55442-1-AP

    IHC staining of mouse kidney using 55442-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 55442-1-AP (SLC12A3/NCCT antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

SLC12A3/NCCT Polyclonal antibody

SLC12A3/NCCT Polyclonal Antibody for WB, IHC, ELISA
Cat No. 55442-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

SLC12A3, NCCT

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected in37°C incubated mouse kidney tissue
Positive IHC detected inmouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

For optimal WB detection with 55442-1-AP, we do not recommend boiling the sample after lysis.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

55442-1-AP targets SLC12A3/NCCT in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse
免疫原

Peptide

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 solute carrier family 12 (sodium/chloride transporters), member 3
别名 SLC12A3, NCCT
计算分子量 114 kDa
观测分子量 150 kDa
GenBank蛋白编号NM_000339
基因名称 SLC12A3
Gene ID (NCBI) 6559
RRIDAB_3743209
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP55017
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SLC12A3 also known as NCCT, NCC, is a sodium-chloride cotransporter (NCC), which mainly functions in the distal convoluted tubules of the kidney and is responsible for the reabsorption of sodium ions and chloride ions (PMID: 35591852). This cotransporter is the target of thiazide diuretics used in the treatment of hypertension. Mutations in SLC12A3 cause Gitelman syndrome(PMID: 8528245, 33462018).

实验方案

Product Specific Protocols
IHC protocol for SLC12A3/NCCT antibody 55442-1-APDownload protocol
WB protocol for SLC12A3/NCCT antibody 55442-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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