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验证数据展示

  • WB analysis of mouse liver using 27938-1-AP

    WB analysis of mouse liver using 27938-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 27938-1-AP (SLC10A6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse heart using 27938-1-AP

    IHC staining of mouse heart using 27938-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 27938-1-AP (SLC10A6 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

SLC10A6 Polyclonal antibody

SLC10A6 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 27938-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Human, Mouse

应用

WB, IHC, ELISA

别名

SLC10A6, SOAT

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse liver tissue
Positive IHC detected inmouse heart tissue, human stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27938-1-AP targets SLC10A6 in WB, IHC, ELISA applications and shows reactivity with Human, Mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 Human, Mouse
免疫原 SLC10A6 fusion protein Ag16609 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 solute carrier family 10 (sodium/bile acid cotransporter family), member 6
别名 SLC10A6, SOAT
计算分子量 377 aa, 41 kDa
观测分子量 70 kDa
GenBank蛋白编号BC107051
基因名称 SLC10A6
Gene ID (NCBI) 345274
RRIDAB_2881014
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ3KNW5
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SLC10A6, also named as SOAT, belongs to the SlC10 sodium-dependent bile acid (BA) transporter family. SLC10A6 is a transporter of steroid sulfates associated with spermatogenesis and fertility. It has been shown that SLC10A6 might be a new anti-proliferative breast cancer target as the SLC10A6 inhibition can affect the proliferation of breast cancer cells. SLC10A6 also plays a key role in hepatic inflammation. 27938-1-AP antibody detects the 70 kDa glycosylated form in SDS-PAGE. (PMID: 26510996, 30186172, 28893621)

实验方案

Product Specific Protocols
WB protocol for SLC10A6 antibody 27938-1-APDownload protocol
IHC protocol for SLC10A6 antibody 27938-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols