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验证数据展示

  • WB analysis using 19909-1-AP

    WB analysis using 19909-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 19909-1-AP (SLC10A3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IHC staining of human pancreas using 19909-1-AP

    IHC staining of human pancreas using 19909-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas using 19909-1-AP (SLC10A3 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human pancreas using 19909-1-AP

    IHC staining of human pancreas using 19909-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas using 19909-1-AP (SLC10A3 antibody) at dilution of 1:50 (under 40x lens).

  • IF Staining of HeLa using 19909-1-AP

    IF Staining of HeLa using 19909-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using SLC10A3 antibody (19909-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4).

SLC10A3 Polyclonal antibody

SLC10A3 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 19909-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, ELISA

别名

P3, DXS253E

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected in37°C incubated A549 cells
Positive IHC detected inhuman pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

19909-1-AP targets SLC10A3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB, IHC
经测试反应性 human, mouse
文献引用反应性human
免疫原 SLC10A3 fusion protein Ag13915 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 solute carrier family 10 (sodium/bile acid cotransporter family), member 3
别名 P3, DXS253E
计算分子量 477 aa, 50 kDa
观测分子量 50 kDa, 48 kDa
GenBank蛋白编号BC004966
基因名称 SLC10A3
Gene ID (NCBI) 8273
RRIDAB_10642950
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP09131
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The solute carrier (SLC) family 10 (SLC10) encodes protein for transporters of various agents, bile acids and steroidal hormones, and SLC01A3 belongs to the SLC10 family. SLC10A3 consists of 477 amino acids, with a calculated mass of approximately 50 kDa.

实验方案

Product Specific Protocols
WB protocol for SLC10A3 antibody 19909-1-APDownload protocol
IHC protocol for SLC10A3 antibody 19909-1-APDownload protocol
IF protocol for SLC10A3 antibody 19909-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

Eur J Med Res

The impact of SLC10A3 on prognosis and immune microenvironment in colorectal adenocarcinoma

Authors - Bangting Wang
View Article
humanWB,IHC

Cancer Cell Int

Integrated profiling identifies DXS253E as a potential prognostic marker in colorectal cancer

Authors - Pu Xing
View Article