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验证数据展示

  • WB analysis using 33396-1-AP

    WB analysis using 33396-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 33396-1-AP (SLAMF6 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse spleen using 33396-1-AP

    IHC staining of mouse spleen using 33396-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 33396-1-AP (SLAMF6 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse spleen using 33396-1-AP

    IHC staining of mouse spleen using 33396-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 33396-1-AP (SLAMF6 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

SLAMF6 Polyclonal antibody

SLAMF6 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 33396-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

mouse

应用

WB, IHC, ELISA

别名

CD352, Ly108, Lymphocyte antigen 108, SLAM family member 6

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse spleen tissue, mouse thymus tissue
Positive IHC detected inmouse spleen tissue, mouse colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:4000-1:16000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

33396-1-AP targets SLAMF6 in WB, IHC, ELISA applications and shows reactivity with mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 mouse
免疫原

CatNo: Eg5249

Product name: Recombinant Mouse SLAMF6/CD352 protein (rFc Tag)

Source: mammalian cells-derived, V37

Tag: C-rFc

Domain: 31-239 aa of NM_030710.2

Sequence: EVSQSSSDPQLMNGVLGESAVLPLKLPAGKIANIIIWNYEWEASQVTALVINLSNPESPQIMNTDVKKRLNITQSYSLQISNLTMADTGSYTAQITTKDSEVITFKYILRVFERLGNLETTNYTLLLENGTCQIHLACVLKNQSQTVSVEWQATGNISLGGPNVTIFWDPRNSGDQTYVCRAKNAVSNLSVSVSTQSLCKGVLTNPPWN

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 SLAM family member 6
别名 CD352, Ly108, Lymphocyte antigen 108, SLAM family member 6
计算分子量36 kDa
观测分子量60 kDa
GenBank蛋白编号NM_030710.2
基因名称 Slamf6
Gene ID (NCBI) 30925
RRIDAB_3742959
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ9ET39-2
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Signaling lymphocyte activation molecule 6 (SLAMF6) (Ly108 in mice, NTB-A or SF2000 in humans) is a homophilic receptor belonging to the superfamily immunoglobulin (Ig) domain-containing molecules (PMID:31199820). SLAMF6 is a type I transmembrane protein with two extracellular immunoglobins (Ig)-like domains and three cytoplasmic tyrosine-based signaling motifs, one of which is immunoreceptor tyrosine-based switch motif (PMID:31315913). SLAMF6 is expressed on a wide variety of immune cells including T cells (also TFH), B cells, NK cells (expressed in humans only), double positive thymocytes, eosinophils, and neutrophils (mouse only) (PMID:11862385).

实验方案

Product Specific Protocols
IHC protocol for SLAMF6 antibody 33396-1-APDownload protocol
WB protocol for SLAMF6 antibody 33396-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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