• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis of EL-4 using 84817-4-RR (same clone as 84817-4-PBS)

    WB analysis of EL-4 using 84817-4-RR (same clone as 84817-4-PBS)

    EL-4 cells were subjected to SDS PAGE followed by western blot with 84817-4-RR (CD229/SLAMF3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84817-4-PBS in a different storage buffer formulation.

  • IHC staining of mouse spleen using 84817-4-RR (same clone as 84817-4-PBS)

    IHC staining of mouse spleen using 84817-4-RR (same clone as 84817-4-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 84817-4-RR (SLAMF3/CD229 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84817-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84817-4-RR

    Affinity and Kinetic Characterization of 84817-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84817-4-RR against Mouse SLAMF3/CD229 were performed. The affinity constant is 2.04 nM.

Mouse CD229/SLAMF3 Recombinant antibody, PBS Only

CD229/SLAMF3 Uni-rAbTM Recombinant Antibody for WB, IHC, Indirect ELISA
Cat No. 84817-4-PBS

产品说明书

CloneNo. 242312E8

宿主/亚型

Rabbit / IgG

种属反应性

mouse

应用

WB, IHC, Indirect ELISA

别名

Lymphocyte antigen 9, Ly-9, Ly9, Cell surface molecule Ly-9, CD229

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

产品信息

84817-4-PBS targets CD229/SLAMF3 in WB, IHC, Indirect ELISA applications and shows reactivity with mouse samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 mouse
免疫原 Recombinant protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 lymphocyte antigen 9
别名 Lymphocyte antigen 9, Ly-9, Ly9, Cell surface molecule Ly-9, CD229
计算分子量73 kDa
观测分子量80-90 kDa, 110 kDa
GenBank蛋白编号NM_001277968.1
基因名称 Ly9
Gene ID (NCBI) 17085
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ01965
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Signaling lymphocytic activation molecule 3 (SLAMF3), also known as CD229 or Ly9, is a type I transmembrane glycoprotein that belongs to the SLAM family receptors (PMID: 10970093; 15905546). It is composed of an extracellular region, which consists of four Ig-like domains, a transmembrane region, and a cytoplasmic tail, which includes two immunoreceptor tyrosine-based signaling motifs (ITSMs) and other tyrosine residues that act as binding sites for the SH2 structural domains (PMID: 8537117; 11369645; 38626547). SLAMF3 is mainly expressed in immune cells, such as T, B, and natural killer cells, and it is involved in the fundamental activation and suppression of these immune cells, as well as the regulation of complex immune networks (PMID: 38626547).