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验证数据展示

  • WB analysis of Jurkat using 10949-2-AP

    WB analysis of Jurkat using 10949-2-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 10949-2-AP (SLAM/CD150 antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

SLAM/CD150 Polyclonal antibody

SLAM/CD150 Polyclonal Antibody for WB, ELISA
Cat No. 10949-2-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, ELISA

别名

CD150, CDw150, IPO 3, SLAM, SLAMF1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inJurkat cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:200-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10949-2-AP targets SLAM/CD150 in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag1386

Product name: Recombinant human SLAMF1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-144 aa of BC012602

Sequence: MDPKGLLSLTFVLFLSLAFGASYGTGGRMMNCPKILRQLGSKVLLPLTYERINKSMNKSIHIVVTMAKSLENSVENKIVSLDPSEAGPPRYLGDRYKFYLENLTLGIRESRKEDEGWYLMTLEKSLHFGYLYFVLGADHDNLQT

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 signaling lymphocytic activation molecule family member 1
别名 CD150, CDw150, IPO 3, SLAM, SLAMF1
计算分子量 37 kDa
观测分子量 70 kDa
GenBank蛋白编号BC012602
基因名称 CD150
Gene ID (NCBI) 6504
RRIDAB_2187822
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ13291
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Signaling lymphocytic activation molecule (SLAM, also known as SLAMF1 or CD150) is a 70-95 kDa transmembrane glycoprotein that belongs to the immunoglobulin gene superfamily. SLAM is constitutively expressed on peripheral blood memory T-cells, T-cell clones, immature thymocytes and a proportion of B-cells, and is rapidly induced on naive T-cells after activation. SLAM is involved in T cell stimulation and cytokine production, and may play an important role in the regulation of immune responses to pathogens.

实验方案

Product Specific Protocols
WB protocol for SLAM/CD150 antibody 10949-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols