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验证数据展示

  • WB analysis using 68547-1-Ig (same clone as 68547-1-PBS)

    WB analysis using 68547-1-Ig (same clone as 68547-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68547-1-Ig (SKIV2L antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68547-1-PBS in a different storage buffer formulation.

  • IHC staining of human placenta using 68547-1-Ig (same clone as 68547-1-PBS)

    IHC staining of human placenta using 68547-1-Ig (same clone as 68547-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 68547-1-Ig (SKIV2L antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68547-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with SKIV2L antibody included
  • IHC staining of human placenta using 68547-1-Ig (same clone as 68547-1-PBS)

    IHC staining of human placenta using 68547-1-Ig (same clone as 68547-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 68547-1-Ig (SKIV2L antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68547-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with SKIV2L antibody included

SKIV2L Monoclonal antibody, PBS Only

SKIV2L Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 68547-1-PBS

产品说明书

CloneNo. 1A9G2

宿主/亚型

Mouse / IgG2a

种属反应性

human

应用

WB, IHC, Indirect ELISA

别名

HLP, Helicase SKI2W, EC:3.6.4.13, DDX13, 1A9G2

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy SKIV2L Ready-To-Use IHC Kit. SKIV2L primary antibody included.

产品信息

68547-1-PBS targets SKIV2L in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag33679

Product name: Recombinant human SKIV2L protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 861-1246 aa of BC015758

Sequence: QVSSNSTSRVFTTLVLCDKPLSQDPQDRGPATAEVPYPDDLVGFKLFLPEGPCDHTVVKLQPGDMAAITTKVLRVNGEKILEDFSKRQQPKFKKDPPLAAVTTAVQELLRLAQAHPAGPPTLDPVNDLQLKDMSVVEGGLRARKLEELIQGAQCVHSPRFPAQYLKLRERMQIQKEMERLRFLLSDQSLLLLPEYHQRVEVLRTLGYVDEVGTVKLAGRVACAMSSHELLLTELMFDNALSTLRPEEIAALLSGLVCQSPGDAGDQLPNTLKQGIERVRAVAKRIGEVQVACGLNQTVEEFVGELNFGLVEVVYEWARGMPFSELAGLSGTPEGLVVRCIQRLAEMCRSLRGAARLVGEPVLGAKMETAATLLRRDIVFAASLYTQ

种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 superkiller viralicidic activity 2-like (S. cerevisiae)
别名 HLP, Helicase SKI2W, EC:3.6.4.13, DDX13, 1A9G2
计算分子量 1246 aa, 138 kDa
观测分子量138-145 kDa
GenBank蛋白编号BC015758
基因名称 SKIV2L
Gene ID (NCBI) 6499
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ15477
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The Ski complex is a multiprotein complex required for exosome-mediated RNA surveillance, including the regulation of normal mRNA and the decay of nonfunctional mRNA. Component of the SKI complex which is thought to be involved in exosome-mediated RNA decay and associates with transcriptionally active genes in a manner dependent on PAF1 complex (PAF1C) [PMID:22444670]. SKIV2L , one component of the Ski complex, acts as an RNA helicase with a role in exosome recruitment or activation. It is involved in the degradation of RNAs by the exosome and may have a role in autophagy [PMID:11719186].