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验证数据展示

  • WB analysis using 19218-1-AP (same clone as 19218-1-PBS)

    WB analysis using 19218-1-AP (same clone as 19218-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 19218-1-AP (SKIL antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19218-1-PBS in a different storage buffer formulation.

  • IHC staining of human endometrial cancer using 19218-1-AP (same clone as 19218-1-PBS)

    IHC staining of human endometrial cancer using 19218-1-AP (same clone as 19218-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 19218-1-AP (SKIL antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19218-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 19218-1-AP (same clone as 19218-1-PBS)

    IF Staining of HepG2 using 19218-1-AP (same clone as 19218-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SKIL antibody (19218-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 19218-1-PBS in a different storage buffer formulation.

  • IP experiment of HepG2 using 19218-1-AP (same clone as 19218-1-PBS)

    IP experiment of HepG2 using 19218-1-AP (same clone as 19218-1-PBS)

    IP result of anti-SKIL (IP:19218-1-AP, 4ug; Detection:19218-1-AP 1:500) with HepG2 cells lysate 1600ug. This data was developed using the same antibody clone with 19218-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

SKIL Polyclonal antibody, PBS Only

SKIL Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 19218-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, IP, Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

19218-1-PBS targets SKIL in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag5282

Product name: Recombinant human SKIL protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 335-684 aa of BC059386

Sequence: YLGTPEEKKLKIILEEMKEKFSMRSGKRNQSKTDAPSGMELQSWYPVIKQEGDHVSQTHSFLHPSYYLYMCDKVVAPNVSLTSAVSQSKELTKTEASKSISRQSEKAHSSGKLQKTVSYPDVSLEEQEKMDLKTSRELCSRLDASISNNSTSKRKSESATCNLVRDINKVGIGLVAAASSPLLVKDVICEDDKGKIMEEVMRTYLKQQEKLNLILQKKQQLQMEVKMLSSSKSMKELTEEQQNLQKELESLQNEHAQRMEEFYVEQKDLEKKLEQIMKQKCTCDSNLEKDKEAEYAGQLAELRQRLDHAEADRQELQDELRQEREARQKLEMMIKELKLQILKSSKTAKE

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 SKI-like oncogene
别名
计算分子量 77 kDa
观测分子量 77 kDa
GenBank蛋白编号BC059386
基因名称 SKIL
Gene ID (NCBI) 6498
RRIDAB_10859824
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP12757
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SKIL, also known as SnoN, is a mediator of the transforming growth factor-β (TGF-β) signaling pathway and exerts pro-oncogenic activity through inhibiting TGF-β/Smad pathway(PMID: 19114989). SKIL is involved in reduction of autophagy and subsequent tumor cell apoptosis in arsenic trioxide-treated promyelocytic leukemia and ovarian carcinoma. In addition, SKIL may regulate cell division or differentiation in response to extracellular signals.

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