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验证数据展示

  • WB analysis using 22938-1-AP

    WB analysis using 22938-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 22938-1-AP (SIX5 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IP experiment of Y79 using 22938-1-AP

    IP experiment of Y79 using 22938-1-AP

    IP result of anti-SIX5 (IP:22938-1-AP, 4ug; Detection:22938-1-AP 1:300) with Y79 cells lysate 2000ug.

  • IHC staining of human kidney using 22938-1-AP

    IHC staining of human kidney using 22938-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 22938-1-AP (SIX5 Antibody) at dilution of 1:200 (under 40x lens).

    Ready-to-use IHC kits available with SIX5 antibody included
  • WB analysis of HeLa using 22938-1-AP

    WB analysis of HeLa using 22938-1-AP

    WB result of SIX5 antibody (22938-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-SIX5 transfected HeLa cells.

  • Featured Product
  • KD/KO验证

SIX5 Polyclonal antibody

SIX5 Polyclonal Antibody for WB, IHC, IP, ELISA
Cat No. 22938-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human and More (1)

应用

WB, IHC, IP, ELISA

别名

Homeobox protein SIX5, DMAHP, DM locus-associated homeodomain protein

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA549 cells, HeLa cells, Y79 cells
Positive IP detected inY79 cells
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy SIX5 Ready-To-Use IHC Kit. SIX5 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

22938-1-AP targets SIX5 in WB, IHC, IP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IP, ELISA Application Description
文献引用应用WB
经测试反应性 human
文献引用反应性human, mouse
免疫原 SIX5 fusion protein Ag18993 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 SIX homeobox 5
别名 Homeobox protein SIX5, DMAHP, DM locus-associated homeodomain protein
计算分子量 739 aa, 75 kDa
观测分子量 100-105 kDa
GenBank蛋白编号BC156983
基因名称 SIX5
Gene ID (NCBI) 147912
RRIDAB_2879186
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8N196
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for SIX5 antibody 22938-1-APDownload protocol
IHC protocol for SIX5 antibody 22938-1-APDownload protocol
IP protocol for SIX5 antibody 22938-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Immunity

An atlas of cells in the human tonsil

Authors - Ramon Massoni-Badosa
View Article
mouseWB

iScience

RBFOX2 regulated EYA3 isoforms partner with SIX4 or ZBTB1 to control transcription during myogenesis

Authors - Hannah J Wiedner
View Article