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验证数据展示

  • IF Staining of HeLa using CL594-66347

    IF Staining of HeLa using CL594-66347

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite®594 SIX2 antibody (CL594-66347, Clone: 1F9G11 ) at dilution of 1:1200, CL488-Phalloidin (green).

  • FC experiment of C2C12 using CL594-66347

    FC experiment of C2C12 using CL594-66347

    1X10^6 C2C12 cells were intracellularly stained with 0.8 ug CoraLite®594 Anti-Human SIX2 (CL594-66347, Clone:1F9G11) (red), or 0.8 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

CoraLite®594-conjugated SIX2 Monoclonal antibody

SIX2 Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL594-66347

产品说明书

CloneNo. 1F9G11

宿主/亚型

Mouse / IgG2b

种属反应性

human, mouse, rat, pig, canine

应用

IF/ICC, FC (Intra)

别名

Homeobox protein SIX2, Sine oculis homeobox homolog 2, SIX homeobox 2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite®594
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inC2C12 cells
For other applications, we recommend the unconjugated version of this antibody, 66347-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:600-1:2400
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-66347 targets SIX2 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat, pig, canine samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human, mouse, rat, pig, canine
免疫原 SIX2 fusion protein Ag2124 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 SIX homeobox 2
别名 Homeobox protein SIX2, Sine oculis homeobox homolog 2, SIX homeobox 2
计算分子量 291 aa, 32 kDa
观测分子量 37 kDa
GenBank蛋白编号BC024033
基因名称 SIX2
Gene ID (NCBI) 10736
RRIDAB_3084706
偶联类型 CoraLite®594 Fluorescent Dye
最大激发/发射波长588 nm / 604 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9NPC8
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The SIX proteins (sine oculis) are a family of homeodomain transcription factors that share a conserved DNA binding domain. Six members (Six1-Six6) of the Six gene family have been identified in mice and humans. SIX2, containing one homeobox DNA-binding domain, is highly expressed in fetal tissues but expression is limited in adult tissues. SIX2 may be involved in limb tendon and ligament development[PMID:21420949]. It has been previously shown that SIX2 is expressed in developing mesenchymal tissue including head and urogenital system at the time of overt midfacial and renal differentiation[PMID: 22282599].

实验方案

Product Specific Protocols
IF protocol for CL594 SIX2 antibody CL594-66347Download protocol
FC protocol for CL594 SIX2 antibody CL594-66347Download protocol
Standard Protocols
Click here to view our Standard Protocols