• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

SIX1 Monoclonal antibody

SIX1 Monoclonal Antibody for IF/ICC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, pig, rabbit, rat

Applications

IF/ICC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

2D7D1

Cat No : 67741-1-Ig

Print datasheet

Synonyms

BOS3, DFNA23, Homeobox protein SIX1, Sine oculis homeobox homolog 1, SIX homeobox 1, SIX1, TIP39

验证数据展示

  • WB analysis using 67741-1-Ig

    WB analysis using 67741-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67741-1-Ig (SIX1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis using 67741-1-Ig

    WB analysis using 67741-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67741-1-Ig (SIX1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IF Staining of A431 using 67741-1-Ig

    IF Staining of A431 using 67741-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using SIX1 antibody (67741-1-Ig, Clone: 2D7D1 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red).

经过测试的应用

Positive WB detected inL02 cells, HeLa cells, rat skeletal muscle tissue, mouse skeletal muscle tissue
Positive IF detected inA431 cells
Planning an IHC experiment? We recommend our IHCeasy SIX1 Ready-To-Use IHC Kit. SIX1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)IF : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67741-1-Ig targets SIX1 in WB, IF, ELISA applications and shows reactivity with Human, mouse, pig, rabbit, rat samples.

Tested Applications IF/ICC, WB, ELISA
Tested Reactivity Human, mouse, pig, rabbit, rat
Immunogen SIX1 fusion protein Ag28570 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name SIX homeobox 1
Synonyms BOS3, DFNA23, Homeobox protein SIX1, Sine oculis homeobox homolog 1, SIX homeobox 1, SIX1, TIP39
Calculated Molecular Weight 32 kDa
Observed Molecular Weight30-35 kDa
GenBank Accession NumberBC008874
Gene Symbol SIX1
Gene ID (NCBI) 6495
RRIDAB_2918510
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ15475
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SIX1 belongs to SIX family that contains a divergent DNA-binding homeodomain and an upstream SIX domain, which may participate both in determining DNA-binding specificity and in mediating protein-protein interactions. It may be involved in limb tendon and ligament development, and specifically expressed in skeletal muscle. SIX1 plays a essential role in the development of numerous organs and shows littlt to no expression in most non-neoplastic adult tissue, yet is overexpression in a number of meoplasms where it increases cell proliferation and survival. The calcualted molecular weight of SIX1 is 32kDa, but modified SIX1 is about 35 kDa.

实验方案

Product Specific Protocols
WB protocol for SIX1 antibody 67741-1-IgDownload protocol
IF protocol for SIX1 antibody 67741-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols