验证数据展示
经过测试的应用
| Positive WB detected in | A2780 cells, HEK-293 cells, HepG2 cells, L02 cells, PC-3 cells, SKOV-3 cells |
| Positive IP detected in | HEK-293 cells |
| Positive IF/ICC detected in | epithelial tumor cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
10709-1-AP targets SIX1 in WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, IP, CoIP, RIP |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | SIX1 fusion protein Ag1044 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | SIX homeobox 1 |
| 别名 | TIP39, SIX homeobox 1, Sine oculis homeobox homolog 1, Homeobox protein SIX1, DFNA23 |
| 计算分子量 | 32 kDa |
| 观测分子量 | 32-35 kDa |
| GenBank蛋白编号 | BC008874 |
| 基因名称 | SIX1 |
| Gene ID (NCBI) | 6495 |
| RRID | AB_2189077 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q15475 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SIX1 belongs to SIX family that contains a divergent DNA-binding homeodomain and an upstream SIX domain, which may participate both in determining DNA-binding specificity and in mediating protein-protein interactions. It may be involved in limb tendon and ligament development, and specifically expressed in skeletal muscle. SIX1 plays a essential role in the development of numerous organs and shows littlt to no expression in most non-neoplastic adult tissue, yet is overexpression in a number of meoplasms where it increases cell proliferation and survival. The calcualted molecular weight of SIX1 is 32kDa, but modified SIX1 is about 35 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for SIX1 antibody 10709-1-AP | Download protocol |
| IP protocol for SIX1 antibody 10709-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cancer Cell Transcriptional Regulation of the Warburg Effect in Cancer by SIX1.
| ||
Adv Sci (Weinh) Transcriptional Regulation of De Novo Lipogenesis by SIX1 in Liver Cancer Cells | ||
Nat Commun Muscle progenitor specification and myogenic differentiation are associated with changes in chromatin topology. | ||
Int J Biol Sci Novel LncRNA LINC02936 Suppresses Ferroptosis and Promotes Tumor Progression by Interacting with SIX1/CP Axis in Endometrial Cancer
| ||
Theranostics O-GlcNAcylation of SIX1 enhances its stability and promotes Hepatocellular Carcinoma Proliferation.
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