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验证数据展示

  • WB analysis using 29729-1-AP

    WB analysis using 29729-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29729-1-AP (SIRT7 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis using 29729-1-AP

    WB analysis using 29729-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29729-1-AP (SIRT7 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IF Staining of HEK-293 using 29729-1-AP

    IF Staining of HEK-293 using 29729-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using SIRT7 antibody (29729-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

SIRT7 Polyclonal antibody

SIRT7 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 29729-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, rat and More (1)

应用

WB, IF/ICC, ELISA and More (2)

别名

SIR2L7, SIR2 like protein 7, NAD-dependent protein deacylase sirtuin-7, EC:2.3.1.286, EC:2.3.1.-

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inCaco-2 cells, HeLa cells, HCT 116 cells, MCF-7 cells, rat liver cells
Positive IF/ICC detected inHEK-293 cells
Planning an IHC experiment? We recommend our IHCeasy SIRT7 Ready-To-Use IHC Kit. SIRT7 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29729-1-AP targets SIRT7 in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human, rat samples.

经测试应用 WB, IF/ICC, ELISA Application Description
文献引用应用WB, IHC, CoIP
经测试反应性 human, rat
文献引用反应性human, mouse, rat
免疫原

CatNo: Ag31794

Product name: Recombinant human SIRT7 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-400 aa of NM_016538

Sequence: MAAGGLSRSERKAAERVRRLREEQQRERLRQVSRILRKAAAERSAEEGRLLAESADLVTELQGRSRRREGLKRRQEEVCDDPEELRGKVRELASAVRNAKYLVVYTGAGISTAASIPDYRGPNGVWTLLQKGRSVSAADLSEAEPTLTHMSITRLHEQKLVQHVVSQNCDGLHLRSGLPRTAISELHGNMYIEVCTSCVPNREYVRVFDVTERTALHRHQTGRTCHKCGTQLRDTIVHFGERGTLGQPLNWEAATEAASRADTILCLGSSLKVLKKYPRLWCMTKPPSRRPKLYIVNLQWTPKDDWAALKLHGKCDDVMRLLMAELGLEIPAYSRWQDPIFSLATPLRAGEEGSHSRKSLCRSREEAPPGDRGAPLSSAPILGGWFGRGCTKRTKRKKVT

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 sirtuin (silent mating type information regulation 2 homolog) 7 (S. cerevisiae)
别名 SIR2L7, SIR2 like protein 7, NAD-dependent protein deacylase sirtuin-7, EC:2.3.1.286, EC:2.3.1.-
计算分子量45KD
观测分子量45 kDa
GenBank蛋白编号NM_016538
基因名称 SIRT7
Gene ID (NCBI) 51547
RRIDAB_2918342
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NRC8
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SIRT7 (NAD-dependent deacetylase sirtuin-7), also known as SIR2L7, is a member of the class IV sirtuin family and is localized to the nucleolus. Expressed throughout the body, SIRT7 associates with rDNA genes where it interacts with histones and acts as a positive regulator of RNA polymerase I (Pol I). SIRT7 is a probable NAD-dependent deacetylase whose expression is upregulated in thyroid carcinoma cells. Overexpression of SIRT7 increases Pol I-mediated transcription, thereby speeding cell growth and contributing to the development of cancer.

实验方案

Product Specific Protocols
WB protocol for SIRT7 antibody 29729-1-APDownload protocol
IF protocol for SIRT7 antibody 29729-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB,CoIP

Acta Pharmacol Sin

JOSD2 alleviates acute kidney injury through deubiquitinating SIRT7 and negativity regulating SIRT7-NF-κB inflammatory pathway in renal tubular epithelial cells

Authors - Ying Zhao
  • KD Validated
View Article
human,ratWB,IHC

Int Urol Nephrol

SIRT7 silencing by miR-152-3p confers cell apoptosis and renal functional impairment induced by renal ischaemia/reperfusion injury

Authors - Yan Wang
View Article