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验证数据展示

  • WB analysis using 12994-1-AP (same clone as 12994-1-PBS)

    WB analysis using 12994-1-AP (same clone as 12994-1-PBS)

    PC-3 cells were subjected to SDS PAGE followed by western blot with 12994-1-AP (SIRT7 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12994-1-PBS in a different storage buffer formulation.

  • IP experiment of PC-3 using 12994-1-AP (same clone as 12994-1-PBS)

    IP experiment of PC-3 using 12994-1-AP (same clone as 12994-1-PBS)

    IP result of anti-SIRT7 (IP:12994-1-AP, 4ug; Detection:12994-1-AP 1:1000) with PC-3 cells lysate 960 ug. This data was developed using the same antibody clone with 12994-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse liver using 12994-1-AP (same clone as 12994-1-PBS)

    IHC staining of mouse liver using 12994-1-AP (same clone as 12994-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 12994-1-AP (SIRT7 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12994-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with SIRT7 antibody included
  • Featured Product
  • KD/KO Validated

SIRT7 Polyclonal antibody, PBS Only

SIRT7 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 12994-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IP, Indirect ELISA

别名

SIR2L7, SIR2 like protein 7, NAD-dependent protein deacylase sirtuin-7, EC:2.3.1.286, EC:2.3.1.-

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy SIRT7 Ready-To-Use IHC Kit. SIRT7 primary antibody included.

产品信息

12994-1-PBS targets SIRT7 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag3656

Product name: Recombinant human SIRT7 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-400 aa of BC017305

Sequence: MAAGGLSRSERKAAERVRRLREEQQRERLRQVSRILRKAAAERSAEEGRLLAESADLVTELQGRSRRREGLKRRQEEVCDDPEELRGKVRELASAVRNAKYLVVYTGAGISTAASIPDYRGPNGVWTLLQKGRSVSAADLSEAEPTLTHMSITRLHEQKLVQHVVSQNCDGLHLRSGLPRTAISELHGNMYIEVCTSCVPNREYVRVFDVTERTALHRHQTGRTCHKCGTQLRDTIVHFGERGTLGQPLNWEAATEAASRADTILCLGSSLKVLKKYPRLWCMTKPPSRRPKLYIVNLQWTPKDDWAALKLHGKCDDVMRLLMAELGLEIPAYSRWQDPIFSLATPLRAGEEGSHSRKSLCRSREEAPPGDRGAPLSSAPILGGWFGRGCTKRTKRKKVT

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 sirtuin (silent mating type information regulation 2 homolog) 7 (S. cerevisiae)
别名 SIR2L7, SIR2 like protein 7, NAD-dependent protein deacylase sirtuin-7, EC:2.3.1.286, EC:2.3.1.-
计算分子量 400 aa, 45 kDa
观测分子量 45 kDa
GenBank蛋白编号BC017305
基因名称 SIRT7
Gene ID (NCBI) 51547
RRIDAB_10644276
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NRC8
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SIRT7 (NAD-dependent deacetylase sirtuin-7), also known as SIR2L7, is a member of the class IV sirtuin family and is localized to the nucleolus. Expressed throughout the body, SIRT7 associates with rDNA genes where it interacts with histones and acts as a positive regulator of RNA polymerase I (Pol I). SIRT7 is a probable NAD-dependent deacetylase whose expression is upregulated in thyroid carcinoma cells. Overexpression of SIRT7 increases Pol I-mediated transcription, thereby speeding cell growth and contributing to the development of cancer.

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